The Stufy of the Effect of Hepatic Copper Content on Signal Intensity in Magnetic Reasonance Images

肝脏铜对肝脏磁共振成像信号强度的影响：分析肝脏铜异常蓄积的LEC大鼠和Wistar大鼠肝脏的信号强度，并研究肝脏信号强度与肝脏铜含量的相关性。LEC大鼠肝脏的T1、T2加权像信号强度与Wistar大鼠无明显差异。铜及铜结合蛋白对肝脏CT衰减值的影响：我们在一个大鼠模型上分析了铜及铜结合蛋白对CT衰减值的影响。5只被认为是肝豆状核变性鼠型的长埃文氏肉桂(LEC)大鼠和28只Wistar大鼠被纳入本研究。将Wistar大鼠分为4组。5只大鼠为正常对照组。12只大鼠结扎胆总管，行ADDI…7例患者在32~60天内出现较多的铜负荷(氯化铜溶液，每天1.8~2.4 mg铜/公斤)。11只大鼠在21-120天期间给予大剂量铜(氮三乙酸铜，每天4 mg铜/公斤)。用原子吸收分光光度法测定肝脏铜、铁含量。检测所有大鼠肝脏平扫CT的CT衰减值，并与肝脏铁、铜含量及溶酶体中与聚合甲硫蛋白对应的地衣素阳性颗粒分级进行比较。肝脏铜值和CT衰减值差异无统计学意义(相关系数=0.056，p=0.96)。肝铁含量与衰减值之间差异有统计学意义(R=0.561，p=0.007)。但CT值与地衣素阳性颗粒分级呈显著正相关(Spearman相关系数Rho=0.755，校正后的Rho为0.738，p=0.0001)。LEC大鼠肝脏CT值与正常对照组无明显差异。结论溶酶体中富含聚合甲硫蛋白(铜结合蛋白)可提高肝脏的CT衰减值，且与肝脏铜含量呈负相关。较少

Effect of hepatic copper on signal intensity in magnetic resonance imaging of the liver : We analyzed the signal intensity of the liver of LEC rats, which have an abnormal copper accumulation in the liver, and of Wistar rats, and studied the correlation between signal intensity of the liver and hepatic copper content. The signal intensity of the liver of LEC rat dose not differ from that of Wistar rats on both T1 and T2 weighted images. We concluded that copper accumulation may not be responsible for the high intensity of HCCs on T1 weighted images.Effect of copper and copper binding protein on CT attenuation value of the liver : We analyzed the effect of copper and copper binding protein on CT attenuation value in a rat model. Five Long-Evan's-cinnamon(LEC)rats, which are thought to be rat form of Wilson's disease, and 28 Wistar rats were included in this study. Wistar rats were divided into 4 groups. Five rats were normal control. In 12 rats, the common bile duct was ligated and addi … More tional copper(cupric chloride solution, daily dose of 1.8-2.4mg copper/kg)was intraperitoneally overloaded in 7 during 32-60 days. High dose copper(cupric nitrilotriacetate, daily dose of 4 mg copper/kg)was given intraperitoneally in 11 rats during 21-120 days. Hepatic copper and iron were measured by atomic absorption spectrophotometry. CT attenuation values at non-enhanced CT of the liver were examined in all rats and compare with hepatic iron and copper content, and grade of orcein positive granules that is thought to be a counter part of polymerized methalothionein in lysosome. The difference hepatic copper and attenuation value at CT was not statistically significant(correlation coefficient=0.056, p=0.96). The difference between hepatic iron content and attenuation value was statistically significant(R=0.561, p=0.007). However, CT value has a strong positive correlation with the grade of orcein positive granules(Spearman's correlation coefficient Rho=0.755, Rho corrected for ties, 0.738, p=0.0001). CT value of the liver of LEC rats did not differ from that of normal control rats. We concluded that CT attenuation value of the liver can be elevated by rich polymerized methalothionein(copper binding protein)in lysosome and has a negative correlation with hepatic copper content. Less