Predictive assay for late radiation injury with fibroblast and lymphocyte

放射治疗是癌症患者非常有用的治疗方式。然而，临床放射治疗存在一些问题。它们是：(1)很难在放疗前预测每种癌症的放射敏感性，(2)不可能在晚期放射损伤发生之前预测。本研究的目的是在放射治疗前发现晚期放射损伤和癌症放射敏感性的预测分析方法。取宫颈癌患者癌组织、皮肤皮下组织及淋巴细胞。从这些组织中培养细胞。对它们进行辐照，并测定每个细胞株的辐射敏感性。肿瘤细胞采用密度测定法，成纤维细胞和淋巴细胞采用普通集落测定法绘制生存曲线。各菌株成纤维细胞的生长速度不同，很难建立有效的检测方法。对于癌细胞，密度测定法的存活率略高于集落测定法，但两者之间存在良好的相关性。另一方面，成纤维细胞和淋巴细胞的放射敏感性与晚期放射损伤的关系尚未确定。原因是：(1)培养成功的病例较少，(2)后期辐射损伤发生率低。我们认为，开发更简便、更灵敏的检测方法，如血清中某些因子的测定是很重要的。今后，辐射损伤分子分析技术的发展将提供更好的检测方法。

Radiotherapy is very useful treatment modality for cancer patients. However, there are several problems to perform clinical radiotherapy. Those are (1) it is very difficult to prospect radiosensitivity of each cancer before radiotherapy, and (2) it is impossible to predict late radiation injury before it happens. This study was performed to develop the predictive assay method to detect late radiation damage and radio-sensitivity of cancer before radiotherapy. Patients with cervical cancer were taken the cancer tissue, the skin subcutaneous tissue and lymphocytes. Cultured cells were established from these tissues. They were irradiated and radiosensitivities for each cell strain were determined. The survival curves were made with densitometry assay method for cancer cells, and with ordinary colony assay method for fibroblasts and lymphocytes. The growth rates of fibroblast were different in each strain and it was very difficult to establish the useful assay method. For cancer cells, the densitometry assay showed slightly higher survival rates than the colony assay, but there was good correlation between these two methods. On the other hand, the relationship between radiosensitivities of fibroblasts and lymphocytes and late radiation injury was not determined. The reasons were (1) number of successful cultures was short, and (2) an incidence of late radiation injury was small number. We concluded that it is important to develop the more easy and sensitive assay method, for example, measurement of some factors in the serum. In future, the developments of molecular analyzes of radiation damage can provide the some better assay methods.