Observation of wet biological specimens by imaging x-ray microscope with the use of zone plates

我们在日本冈崎的UVSOR安装了带有双波带片的成像X射线显微镜，并应用它观察了各种生物标本的干燥和潮湿。用波带板作为测试样品，我们观察到了64 nm的精细区带，这非常接近理论分辨率极限（55 nm）。作为干样品，我们观察到了硅藻、肌原纤维、蛤单胞菌等样品，这些样品的图片见本活动报告的主要部分。由于束流时间的限制，湿标本只能观察到肌原纤维或肌原纤维碎片的图像，我们认识到今后还有几个问题需要解决，主要是关于聚光区板;样品没有被均匀地照射，可能是由于聚光器波带片与微波带片的光学参数不匹配，或者由于致密波带片从设计值变形。

We have mounted imaging x-ray microscope with two zone plates at UVSOR(Okazaki, Japan), and applied it to observe various bio-specimens dry or wet. With a zone plate as a test specimen, we observed zones as fine as 64 nm, which was very close to theoretical resolution limit (55nm).As dry specimens, we observed specimens such as diatom, myofibril, clamidomonas, of which pictures are shown in the main volume of the present activity report. As wet specimen, we only observed an image of myofibril or its fragment, as the limitation of usable beam time.we realized that we still have several probelems to be solved in future, mainly concerning the condenser zone plate ; specimens are not illuminated uniformly, probably due to the mismatching of optical parameters of condenser zone plate to the micro zone plate, or due to the deformation of the consenser zone plate from the designed values.