STUDY OF BIODEGRADABLE MATERIALS FOR ANTI-ADHESIVE MEMBRANE IN VIVO

在屈肌腱单独损伤的情况下，可以通过早期控制性活动来防止粘连。但对于严重损伤的肌腱和腱鞘缺损，需要4周的制动。交联明胶是为防粘连膜而研制的。本研究观察了该膜在大鼠体内8周的反应和在鸡肌腱手术后4周内对减少粘连的作用。材料与方法：明胶在真空、低压、150 ℃条件下交联24小时。用两种不同浓度内毒素（内毒素（+）、（-））的蒸馏水制备。将明胶片皮下植入大鼠背部。分别于植入后2、4、7、10、14 d处死动物，行组织学观察。术后4、7、10天内毒素阳性标本周围有白细胞浸润。但在内毒素阴性的鸡标本周围，直到14天才出现反应。左侧第三脚趾的腱鞘被切除，屈肌腱被切断，骨底受伤。将肌腱缝合并用明胶膜包裹。在右脚趾上，肌腱单独缝合。术后2周和4周处死鸡。对足趾进行组织学观察和动态观察。在组织学研究中，使用膜有效地消除了粘连。在动态研究中，两组在2周和4周时无显著差异。

In the case of flexor tendon injury alone, adhesion can be prevented by early controlled mobilization. But immobilization for 4 weeks is required for a badly injured tendon with the tendon sheath defects. Crosslinked gelatin was developed to apply such cases as anti-adhesive membrane. In this study we observed the reactions of the membrane for 8 weeks in rats and the effects on reduction of adhesion for 4 weeks after tendon surgery in chickens.Materials and methods : Gelatin was crosslinked under the condition of vacuum, low pressure and 150ﾟC for 24 hours. It was made using two kinds of distillled water of different concentrations of endotoxin (endotoxin (+), (-)). The gelatin sheets were implanted in the back of rats subcutaneously. They were sacrificed at 2,4,7,10 and 14 days after implantation and histological study was performed. At 4,7 and 10 days postoperatively, infiltration of leukocyte was seen in around endotoxin (+) specimen. But its reaction was not seen around endotoxin (-) specimen until 14 days.Then the chickens aged 3 to 4 months were used. On the left third toe, the tendon sheath was removed, the flexor tendon was cut and the bony floor was injured. The tendon was sutured and wrapped around with the gelatin membrane. On the right toe, the tendon was sutured alone. The chickens were sacrificed at 2 and 4 weeks postoperatively. The toes were studied histologically and dynamic study was also performed. In the histological study, adhesion was eliminated effectively by using the membrane. In the dynamic study, there was no significant difference between the two groups at 2 and 4 weeks.