Effects of paraasite larva excretory-secretory products on hemocytes of intermediatehost snail

用21种异硫氰酸荧光素标记的凝集素（Con A、DBA、PNA、RCA 120、SBA、UEA-I、WGA、GSL-I、LCA、PHA-E、PHA-L、PSA、SJA、S-WGA、GSL-II、DSL、ECL、Jacalin、LEL、STL、VVL）对中间宿主钉螺（Biomphalaria glabrata）血细胞表面糖进行了表征。光滑B血中有粒细胞和透明细胞两种血细胞类型，其中粒细胞是最直接参与消灭寄生虫的细胞。凝集素可识别的α-甲基甘露糖苷结合两种血细胞类型，凝集素可识别的N-乙酰-D-葡糖胺结合透明质细胞。感染副鳗棘口吸虫后，血细胞凝集素结合模式没有改变。使用Caulter EPICS Profile流式细胞仪进行前向和侧向散射测量。新鲜抽取的血细胞的大小分布是单峰的和连续的，没有离散的亚群，并不受年龄或感染副粘多糖。而血细胞的侧向散射随钉螺体积的增大和感染副粘多糖而减小。使用DNA特异性碘化丙啶荧光测量细胞周期每个阶段中血细胞的比例。感染钉螺血细胞DNA含量较低的比例增加。

Twenty-one fluorescein isothiocyanate-conjugated lectins (Con A,DBA,PNA,RCA120, SBA,UEA-I,WGA,GSL-I,LCA,PHA-E,PHA-L,PSA,SJA,S-WGA,GSL-II,DSL,ECL,Jacalin, LEL,STL,VVL) were used to characterize surface carbohydrates of intermediate snail host (Biomphalaria glabrata) hemocytes. Two haemocyte types, granulocytes and hyalinocyte was described from B.glabrata blood, and granulocytes are the cell most derectory involoved in destruction of parasites. Lectins inhibitable alpha-methylmannoside bound to both hemocyte types, and lectins inhibitable N-acetyl-_D-glucosamine bound to hyalinocytes. There was no change in lectin-binding patterns by infection with Echionostoma paraensei.Flow cytometric analysis was performed on hemocytes of snail host. Forward and side scatter measurements were made using a Caulter EPICS Profile flow cytometer. The size distribution of freshly drawn hemocytes was unimodal and continuous, with no discrete subpopulations, and is not affected by age or by infection with E.paraensei. However, the side scatter of hemocytes was decteased with increasing of snail size and infection with E.paraensei. The proportion of hemocytes in each stage of cell cycle was measured using DNA-specific propidium iodide fluorescence. The proportion of hemocytes with lower DNA content increased in infected snails.