The Time-Dependent Expression of Proliferating Cell Marker in Skin Wounds

伤口愈合伴随着细胞增殖。近年来，增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）作为细胞分裂的标志物的研究报道较多。本文应用PCNA单克隆抗体对大鼠皮肤切割伤进行免疫组化研究，结果表明：正常大鼠皮肤的表皮基底细胞（细胞核）绝大多数呈PCNA阳性反应，故生前伤与死后伤难以区分。作者观察了创伤愈合过程中PCNA阳性细胞在创面周围的分布。伤后6小时，创面表面可见少量PCNA阳性细胞。1天皮损内可见大量成纤维细胞和毛细血管内皮细胞染色阳性。伤后4d左右阳性细胞数达到高峰。伤后5-6天肉芽组织中虽仍有较多细胞，但PCNA阴性细胞增多。PCNA阳性细胞的相对减少似乎与胶原的增加有关。PCNA染色可清楚地观察到新鲜创面细胞分裂的开始和肉芽组织细胞分裂的高峰。

Wound healing accompanies cell proliferation. Recently, there are many reports that proliferating cell nuclear antigen (PCNA) is useful as the indications of cell division. In this paper, incised skin wounds of rats were studied by immunohistochemistry using monoclonal antibody against PCNA.As the great majority of epidermal basal cells (nuclei) of uninjured rat skin showed PCNA positive reaction, it is difficult to differentiate between antemortem and postmortem wounds. The authors observed the distribution of PCNA positive cells around wound area during wound healing. A few of PCNA positive cells appeared on the surface of 6-hr vital wounds. In oneday-old skin lesion, a number of fibroblasts and endothelial cells of capillaries stained positive. The number of positive cells reached a peak in about 4 days after wounding. Though there were still a good many of cells in granulation tissue with a wound age of 5-6 days, the number of PCNA negative cells increased. The relative decrease of PCNA positive cells seemed to be associated with the increase of collagen. Using PCNA staining, it can be easily followed the beginning of cell division on fresh wound surface and the highest peak of it in granulation.