EVALUATION OF EFFECT OF SODIUM PENTOSAN POLYSULFATE (SPP) AND CITRATE ON STONE FORMATION IN HYPEROXALURIC RATS BY RADIOLUMINOGRAPHY USING ^<14>C-OXALATE

用放射发光技术研究了多聚戊聚糖硫酸钠(SPP)和柠檬酸对喂饲维生素B6缺乏饲料所致高草酸尿大鼠草酸钙结石形成的影响。将高草酸尿大鼠分为4组。第一组大鼠只喝自来水作为对照。第2组(SPP 2~4w组)大鼠停药2周后隔日注射SPP 10 mg，连续2周。第3组(SPP 0~4w组)隔日给予等量SPP，连续4周。最后一组大鼠(Cit组)在自来水中加入60 mM柠檬酸盐维持4周。4周后静脉注射14&GT；C-草酸(7.4MBq/kg)，18h后处死大鼠，行双侧肾切除。用切片机将肾脏切成40微米厚。每个肾脏标本都直接与成像板接触。~(14)&gt；C-oxal…的放射性用光激励发光法测定每个显像板上的ATE。与常规放射自显影技术相比，放射照相技术可以更准确地测量高速缓存肾切片中的放射性。此外，放射照相法测量的放射性与液体闪烁计数器法测量的放射性有很好的相关性。分析每只动物的10个放射免疫图谱，并计算~(14)&GT；C-草酸沉淀物的总数和总的被测放射性。对照组大鼠肾乳头内可见草酸钙沉积，提示草酸钙结石的形成。在其他三组中，~(14)&GT；C-草酸盐析出物的总数和总放射性均显著低于对照组。三组沉积物的最大放射性活度均显著低于对照组。这些结果表明，SPP和柠檬酸都是抑制尿路结石形成的有效药物。较少

Using radioluminographic techniques, we studied the effect of sodium pentosan polysulfate (SPP) and citrate on calcium oxalate stone formation in hyperoxaluric rats induced by feeding vitamin B6 deficient diet. Hyperoxaluric rats were divided into four groups. Rats in the first group were given only tap water as the control. Rats in the second group (SPP 2-4w group) had no administration for 2 weeks and after that time were injected with 10mg SPP every other day for 2 weeks. The third group (SPP 0-4w group) received the same dose SPP every other day for 4 weeks. And rats in the last troup (Cit group) were maintained with 60mM citrate in tap water for 4 weeks. At 4 weeks all rats were injected with ^<14>C-oxalate intravenously (7.4MBq/kg) and 18 hours after the injection they were killed and immediately nephrectomized bilaterally. Kidneys were sliced 40mum in thickness using a microtome. Each kidney specimen was directly in contact with an imaging plate. The radioactivity of ^<14>C-oxal … More ate in each imaging plate was measured by photostimulated luminescence method. Radioactivity in cach kidney slice could be measured more accurately by radioluminographic techniques than conventional autoradiographic studies. Moreover, the radioactivity measured by radioluminography correlated excellently with that of liquid scintillation counter method. Ten radioimmunograms were analyzed in each animal and both the total number and total detected radioactivity of deposits of ^<14>C-oxalate were calculated. The maximum radioactivity of deposits in each rat was also measured.In control group, deposits of ^<14>C-oxalate which indicated calcium oxalate stone formation were induced in the renal papilla. In the other three groups, the total number and total detected radioactivity of deposits of ^<14>C-oxalate were significantly decreased in comparison with the control. The maximum radioactivity of deposits in these three groups was significantly lower than that of the control group, respectively. These results indicate that both SPP and citrate must be effective inhibitory agents on urinary stone formation. Less