Effects of PBSC reinfusion on tumor immunity in patients with urologic malignancies

外周血祖细胞(PBSC)回输促进了泌尿系肿瘤和血液系统恶性肿瘤患者清髓化疗的进展。通过CD34抗原的表达，可以确定人骨髓和外周血中的虚拟集落形成细胞是祖细胞。通常认为，回输PBSC中CD34阳性细胞的数量与造血恢复有关。利用流式细胞仪分析，我们首先建立了一种检测外周血和PBSC收获细胞中CD34阳性细胞浓度的技术(发表在BiothRapy上)。我们检测CD34阳性细胞的技术已经建立，并与克隆形成试验(发表在Igakukensa上)的结果相关联。另一方面，PBSC收获的细胞含有大量巨噬细胞。最近有报道称，这种巨噬细胞强烈分泌刺激造血的细胞因子。其次，我们研究了PBSC回输对患者免疫调节的影响。CD69抗原是一种早期活化抗原，也是一种与NK基因家族复合体相关的抗原。我们证实CD69抗原表达的变化是外周血干细胞移动化(ISBT)的良好指标。然而，在这个免疫学问题变得清晰之前，似乎还需要进一步的研究。总之，我们建立了检测外周血和PBSC中CD34阳性细胞的技术。循环单核细胞表面CD69抗原表达的变化可能反映了患者的免疫反应。

Peripheral blood progenitor cells (PBSC) reinfusion has contributed to the advance of myeloablative chemotherapy in patients with urologic cancers as well as hematological malignancies. Virtually colony forming cells as progenitors in human bone marrow and peripheral blood can be identified by the expression of the CD34 antigen. It is commonly believed that number of CD34 positive cells in PBSC reinfusion have been correlated with hematopoieticrestore. Using flow cytometric analysis, first we established a technique for detecting CD34 positive cells' concentration in peripheral blood and PBSC harvesting cells (published in Biothrapy). Our technique for detecting CD34 positive cells had been well established and correlated with the results of colony formation assay (published in Igakukensa).On the other hands, PBSC harvested cells contained a number of macrophages. It was recently reported the such macrophages strongly secleted cytokines for stimulating hematopoiesis. We secondly investigated on the effect of PBSC reinfusion to the immune-modulation in patients. CD69 antigen that is an early activating antigen and also an antigen related with NK-gene family complex was identified by dual color flow cytometric analysis on cytokine seclated circulating monocytes in PBSC harvesting cells (ISBT). We confirmed change in CD69 antigen expression to be a good indicator of PBSC moblization (ISBT). However, before this immunological issue become clear, it seemed further investigations will be required. In conclusion, our establishment technique for detecting CD34 positive cells in peripheral blood and PBSC harvesting cells is well established. Change in CD69 antigen expression on circulated monocytes might demonstrate some immunological reaction in patients.