Epidemiologic study on HPV infection for protecting generation of cervical cancer

(a)宫颈癌组织中人乳头瘤病毒18型的基因组结构研究有报道称，人乳头瘤病毒（HPV）18阳性的宫颈癌比其他HPV类型的宫颈癌预后差。为了更好地了解HPV 18阳性癌症的侵袭性，我们试图找到HPV 18和HPV 16携带宫颈癌之间基因组组织的差异。应用聚合酶链反应（PCR）技术，对9例HPV 18阳性和31例HPV 16阳性的宫颈癌组织进行E1和E2基因扩增。9例HPV 18阳性癌中有3例两个早期基因中至少有一个缺失，而31例HPV 16阳性癌中两个PCR均阳性（P<0.05）。然后，我们通过15个连续的聚合酶链反应分析了9个HPV 18阳性的癌症，这些聚合酶链反应共同覆盖了整个HPV 18基因组。除了E1或E2基因缺失的三个外，一个E5和L2基因缺失，一个长控制区插入 ...更多信息 .基因组组织的频繁改变，特别是E1或E2基因的优先缺失，可能与HPV 18阳性宫颈癌更具侵袭性的特性有关。(b)多型人乳头瘤病毒与外阴肿瘤的相关性本研究的目的是阐明多型生殖器人乳头瘤病毒（HPV）与外阴肿瘤的相关性。我们已经检查外阴肿瘤的聚合酶链反应（PCR）方法，可以检测几乎所有的生殖器HPV类型敏感（L1-PCR）。53例外阴尖锐湿疣中有52例（98.1%）检测到HPV 6、11、16、18、52、53、56和68型，6例外阴上皮内瘤变中有2例（16和18型），11例外阴浸润性癌中有8例（72.7%）检测到HPV 6、16、18、51和56型。外阴尖锐湿疣中HPV 52、53、56和68型及外阴癌中HPV 51和56型的检出为首次报道。这表明更多的HPV类型与外阴肿瘤的产生有关，而不是到目前为止所认为的。少

(a) Genomic Organization of Human Papillomavirus Type 18 in Cervical Cancer SpecimensIt has been reported that human papillomavirus (HPV) 18-positive cervical cancers have a poorer prognosis than those with the other HPV types. To gain a better understanding of the aggressive property of HPV 18-positive cancers, we tried to find the difference in genomic organization between HPV 18 and HPV 16 harboring cervical cancers. We amplified E1 and E2 genes from 9HPV 18-positive and 31 HPV 16-positive cervical cancers by polymerase chain reaction (PCR). At least one of the two early genes were missing in 3 out of 9 HPV 18-positive cancers, while both PCRs were positive in all 31 HPV 16-positive cancers (P<0.05). We then analyzed the 9 HPV18-positive cancers by 15 contiguous polymerase chain reactions covering collectively the whole HPV18 genome. In addition to the three with a deletion of the E1 or E2 gene, one had a deletion in E5 and L2 genes and one had a insertion in the long control region … More . The frequent alterations in genomic organization, especially preferential deletion of the E1 or E2 gene, may be related to the more aggressive properties of HPV 18-positive cervical cancers.(b) Association of Multiple Human Papillomavirus Types with Vulvar NeoplasiasThe purpose of this study was to clarify the association of multiple genital human papillomavirus (HPV) types with vulvar neoplasias. We have examined vulvar neoplasias by the polymerase chain reaction (PCR) method which can detect almost all genital HPV types sensitively (L1-PCR). Eight HPV types (types 6,11,16,18,52,53,56 and 68) were detected in 52 of 53 (98.1%) vulvar condylomas ; two HPV types (types 16 and 18) in all 6 vulvar intraepithelial neoplasias grade 3 ; five HPV types (types 6,16,18,51 and 56) in 8 of 11 (72.7%) invasive vulvar carcinomas. Detection of HPV types 52,53,56 and 68 in vulvar condylomas and that of HPV types 51 and 56 in vulvar carcinomas are first examples ever reported. It suggests that more HPV types are associated with the generation of vulvar neoplasias than thought so far. Less