Transforming growth factor-beta1 coated beta-tricalcium phosphate pellets stimulate healing of experimental bone defects of rat calvariae

为了评价转化生长因子-β1包被的β-磷酸三钙颗粒移植修复大鼠双侧颅骨缺损的效果，我们用HE染色进行了光镜观察，并检测了成骨细胞和破骨细胞的碱性磷酸酶(ALPase)和抗酒石酸酸性磷酸酶(TRACPase)活性。用激光共聚焦扫描显微镜(CLSM)对新生骨进行形态测量。H-E切片显示，在接受涂有转化生长因子-β1涂层的β-磷酸三钙颗粒的部位(实验部位)，比只接受β-磷酸三钙颗粒的部位(对照部位)有更强的新骨形成。第4周，在实验部位观察到新的骨斑点：从周围和内部都有骨形成。TRACPase阳性细胞和ALPase阳性细胞在实验部位的分布频率均高于对照部位。计算机化的CLSM像素图像分析计算的骨形成率证实了上述发现(2周时9.3(sy.+-.)1.7%vs.3.4(sy.+-.))0.8%；4周时39.8(sy.+-.)10.9%vs.11.8(sy.+-.)2.1%；转化生长因子-β1包裹的β-tcp小丸vs.综上所述，包被转化生长因子-β1的β-磷酸三钙颗粒是一种推荐的促进骨形成的人工骨移植材料。

In an attempt to assess the effect of TGF-beta1-coated beta-TCP pellet grafting on bilateral defects created in rat calvariae, we carried out morphological observations by light microscopy using hematoxylincosin (H-E) staining for histology and detection of alkaline phosphatase (ALPase) and tartrateresistant acid phosphatase (TRACPase) activities for ostcoblastic and osteoclastic cell distribution. Confocal laser scanning microscopy (CLSM) was performed for morphometry of newly formed bone. H-E sections disclosed stronger new bone formation in sites receiving TGF-beta1-coated beta-TCP pellets (experimental sites) than in beta-TCP pellets alone (control sites). On week 4, new bone spots in experimental sites were observed : bone formation occurred from both the periphery and within. The frequency of scquentially arranged TRACPase-positive cells and ALPase-positive cells in experimental sites was higher than in control sites. The bone formation rate calculated by computerized CLSM pixel image analysis confirmed the above findings (9.3(]SY.+-.])1.7% vs. 3.4(]SY.+-.])0.8% on week 2 and 39.8(]SY.+-.])10.9% vs. 11.8(]SY.+-.])2.1% on week 4 ; TGF-beta1-coated beta-TCP pellets vs. beta-TCP pellets). To conclude, TGF-beta1-coated beta-TCP pellet is a recommended synthetic bone graft which promotes bone formation.