MORPHOLOGICAL STUDY ON THE SURVIVAL AND AXONAL GROWTH OF TELENCEPHALIC AND BRAINSTEM NEURONS BY S-100beta.

具有遗传性多指（Polydactyly名古屋; Pdn）的小鼠品系的纯合子显示脑中S-100 β显著降低。为了阐明S-100 β生成延迟对单胺能神经元系统和胶质细胞发育的影响，我们对酪氨酸羟化酶（TH）、5-羟色胺（5-HT）、S-100 β和胶质细胞酸性蛋白（GFAP）进行了免疫组织化学研究。在所有类型的小鼠中，S-100 β阳性细胞和多巴胺能纤维广泛分布于整个脑中，多巴胺能细胞体位于脑干中。而Pdn/Pdn小鼠海马和尾背侧皮质S-100 β阳性细胞和多巴胺能纤维明显减少。此外，在Pdn/Pdn组大鼠大脑皮质和海马中，GFAP阳性细胞和纤维分布异常。TH神经元和TH纤维的分布无明显差异。目前的结果表明， ...更多信息 Pdn突变小鼠中β 1能纤维的缺陷与该突变小鼠星形胶质细胞中S-100 β的缺陷有关。为进一步阐明不同脑型靶组织对多巴胺能神经元发育的影响，将Pdn/Pdn或+/+新生小鼠的中缝核神经元与+/+或Pdn/Pdn新生小鼠的海马或皮质共培养。结果表明，与+/+脑靶组织（海马和皮质）共培养可促进Pdn/Pdn和+/+两种基因型多巴胺能神经元的发育。在以Pdn/Pdn脑作为靶组织来源的共培养物中未观察到这种效应。本研究结果支持了Pdn突变小鼠的多巴胺能神经纤维发育缺陷与星形胶质细胞S-100 β缺陷有关的观点，并提示S-100 β是多巴胺能生长因子。少

The homozygote of a mouse strain with genetic polydactyly (Polydactyly Nagoya ; Pdn) shows significant decrease of S-100beta in the brain. in order to clarify the effects of the retarded production of S-100beta on the development of monoaminergic neuron systems and glia cells, immunohistochemical studies of tyrosine hydroxylase (TH), serotonin, S-100beta and glial fibrillary acidic protein (GFAP) was performed. In all types of mice, S-100beta positive cells and serotonergic fibers were widely distributed throughout the brains and serotonergic cell bodies were located in the brainstem. However, the hippocampus and caudo-dorsal cortex of Pdn/Pdn mouse were markedly reduced in S-100beta positive cells and in serotonergic fibers. Furthermore, abnormal distribution of GFAP positive cells and fibers were observed in the cortex and hippocampus of Pdn/Pdn brain. No differences were seen in the distribution of TH neurons and fibers distribution. The present result suggests that the developmenta … More l defect of serotonergic fibers in the Pdn mutant mouse is correlate to the deficiency of S-100beta in the astrocyte of this mutant. To further clarify the effects of target tissues from different type brains on the development od serotonergic neurons, raphe neurons from Pdn/Pdn or+/+ newborn mice were co-cultured with hippocampus or cortex of +/+ or Pdn/Pdn newborn mice. The growth of serotonergic neurons in the mesencephalic raphe tissue dissociated cultures was estimated by measuring the specific uptake of [^3H] 5HT.The development of both genotypes (Pdn/Pdn and +/+) of serotonergic neurons was enhanced by co-cultures with target tissue (hippocampus and cortex) of +/+ brain. This effect was not observed in the co-cultures with Pdn/Pdn brain as a source of target tissue. The present results support the idea that the developmental defect of serotonergic fibers in the Pdn mutant mouse is correlate to the deficiency of S-100beta in the astrocyte of this mutant, and suggest that S-100beta is a serotonergic growth factor. Less