Mechanism of recovery of autonomic function in the pelvic ganglia following pelvic nerve transection

用电压敏感染料和光电二极管阵列检测系统研究了盆神经切断后盆神经节的可塑性。实验前2-4周行单侧盆神经切断术。戊巴比妥深度麻醉后，取出两侧盆神经节，用台氏液孵育。将神经节用电压敏感染料暴露30分钟，并使用Deltalon 1700（Fuji Film Co，Tokyo）测量其亮度。用计算机系统对数字化信号进行分析。当亮度变化小于对照的0.01%时，颜色保持在绿色。黄色显示0.05%的变化，红色显示超过0.01%的变化。在对照盆神经节中，亮度在连续测量期间没有变化，并且计算机演示显示为绿色。施加KCl（50 mM-150 mM）在0.9 s内使颜色变为红色并且颜色恢复为绿色（对照）。红色区域随KCl剂量的增加而增加。应用ACh（3.3 μ M-50 μ M）也使颜色变为红色，这与KCl不完全匹配（似乎比KCl面积小，但独立）。在分散性神经节内记录到自发放电，放电起始于神经节内的几个点，并向周围区域移动。每0.3 - 0.5s发生一次击发，这对河豚毒素敏感。应用KCl和ACh也激活神经节细胞。KCl兴奋阈值与对照组相同，ACh兴奋阈值为对照组的1/5 ~ 1/10。提示盆神经切断后，盆神经节内形成了新的兴奋网络系统，可通过腹下神经的少量输入兴奋神经节内的大部分细胞。

Plasticity of the pelvic ganglia in the rat following pelvic nerve transection has been studied using voltage sensitive dye and photodiode array detecting system. Unilateral transection of the pelvic nerve was performed 2-4 weeks prior to the experiments. After deeply anesthetized with nembutal, both side of the pelvic ganglia were removed out and incubated with Tyrode's solution. The ganglia were expose with voltage sensitive dye for 30 min and measuring its brightness using Deltalon 1700 (Fuji Film Co, Tokyo). Diditalized signal were analyzed with computer system. When the brightness change were than 0.01% of the control, the color stayd in green. Yellow showed 0.05% change and red showed more than 0.01% change. 256 color difference will be presented on the screen.In control pelvic ganglia, the brightness did not change over the period of continuous measurements and computer demonstration showed green. Application of KCl(50mM - 150mM) changed the color to red and the color return to green(control) in 0.9s. The red area increased by KCl doses. Application ACh(3.3muM - 50muM) also changed the color to red which is not completely match with KCl(seems less area than KCl, but independent). The excitation return to control in 2S.In the decentralized ganglia, spontaneous firing was recorded, The firing started few point in the ganglia and moved to the surrounded area. The firing occurred every 0.3 - 0.5s, and this is sensitive to tetrodotoxin. Application of KCl and ACh also activate the ganglia cells. Threshold of KCl excitation as same as control but ACh was 1/5 - 1/10 of the control.These data suggest new excitation network system was developed following pelvic nerve transection, and this could contribute to excite most of cells in the ganglia by small input from hypogastric nerve.