Mass Spectrometry Platform (Part II): Ion Mobility Q-TOF Mass Spectrometer with Nano-HPLC System

本文描述的质谱分析平台将用于最近成立的由Sinz教授领导的“结构质谱分析中心”（CSMS）的蛋白质结构分析。该设备将用于开展与马丁·路德大学自然科学学院和医学院的合作研究项目，以及RTG 2467“内在无序蛋白质-分子原理，细胞功能和疾病”，以研究三维蛋白质结构并描述蛋白质-蛋白质相互作用。两种LC/MS系统都必须满足所有要求，以保证对蛋白质进行稳健、高通量的分析。两个离子迁移率Q-TOF质谱仪项目将有明确的分离：一个LC/MS系统将用于交联/质谱实验，另一个LC/MS系统将用于H/D交换实验。这将允许获得蛋白质和蛋白质复合物的三维结构分析的补充数据，直到系统范围的蛋白质网络分析。建立csm将进一步加强MLU Halle-Wittenberg对蛋白质质谱的研究重点。

The mass spectrometry platform described herein will be used for protein structure analysis in the recently established “Center for Structural Mass Spectrometry” (CSMS) headed by Prof. Sinz. The equipment will serve to conduct cooperative research projects with the Faculty of Natural Sciences I and the Medical Faculty at the Martin Luther University Halle-Wittenberg as well as within the RTG 2467 “Intrinsically Disordered Proteins – Molecular Principles, Cellular Functions, and Diseases” to investigate three-dimensional protein structures and to delineate protein-protein interactions. Both LC/MS systems have to fulfill all requirements to guarantee a robust, high-throughput analysis of proteins. There will be a clear separation of projects for the two ion mobility Q-TOF mass spectrometers in the CSMS: One LC/MS system will be employed for cross-linking/MS experiments, while the second LC/MS system will be used for conducting H/D exchange experiments. This will allow obtaining complementary data for the three-dimensional structural analysis of proteins and protein complexes up to system-wide protein network analyses. Establishing the CSMS will further strengthen the research focus on protein mass spectrometry at the MLU Halle-Wittenberg.