Consequences of an Ascaris - Salmonella coinfection on macrophage functions and the anti-Salmonella antibody response in pigs

计划中的项目将从功能上分析猪的两种常见人畜共患病病原体猪蛔虫和鼠伤寒沙门氏菌混合感染的免疫学后果。我们计划将重点放在蠕虫诱导的巨噬细胞上，因为这些细胞是蠕虫免疫调节的目标细胞，同时也是沙门氏菌繁殖和持久性的重要细胞。我们推测，蛔虫感染通过调节巨噬细胞强烈影响沙门氏菌感染。此外，还将描述在猪同时感染蛔虫的情况下产生抗体的浆细胞和猪对沙门氏菌的抗体反应。蛔虫对抗沙门氏菌抗体反应的调节对于诊断沙门氏菌在养猪场和屠宰过程中的人畜共患病风险具有重要意义。总之，该项目的总体目标是破译人畜共患病病原体对猪细胞和体液免疫反应的影响，并检测由此改变的细菌病原体的持久性。

The planned project will functionally analyze the immunological consequences of a coinfection of two common zoonotic pathogens, Ascaris suum and Salmonella Typhimurium in pigs. We plan to focus on helminth-induced macrophages, as these are target cells of helminth immunomodulation and at the same time important cells for salmonella reproduction and persistence. We hypothesize that the Ascaris-infection strongly influences the Salmonella infection via modulated macrophages. In addition, antibody-producing plasma cells and the pig antibody response against Salmonella will be depicted in presence of a concomitant Ascaris-infection in pigs. A modulation of the anti-Salmonella antibody response by Ascaris is of great importance for the diagnostic of Salmonella in pig farming and at slaughter due to the resulting zoonotic risk.In conclusion, the overall aim of the project is to decipher the impact of a coinfection of the zoonotic pathogens A. suum und S. Typhimurium on the cellular and humoral immune response in pigs and to detect the consequently changed persistence of the bacterial pathogen.