Conformational switches in viral regulatory proteins (B15*)

蛋白HBx对于B型肝炎病毒的复制是必需的，因为它使得病毒DNA能够转录。HBx的结构未知。翻译后修饰、金属结合和构象转换进一步调节其在溶液中的折叠。我们将通过NMR与生物物理方法的结合，特别是SAXS，FRET和分子动力学模拟，阐明HBx的结构和动力学。此外，我们将分析HBx与其在感染肝细胞中的人靶蛋白的相互作用。

The protein HBx is essential for the replication of Hepatitis B virus as it enables the transcription of the viral DNA. The structure of HBx is unknown. Posttranslational modifications, metal binding and conformational switches further modulate its fold in solution. We will elucidate structure and dynamics of HBx via integration of NMR with biophysical methods, in particular SAXS, FRET and molecular dynamics simulations. Furthermore, we will analyse the interaction of HBx with its human target proteins in infected hepatocytes.