STED Superresolution and Confocal Microscope

Charité - Universitätsmedizin柏林的Campus Benjamin富兰克林（CBF）的用户联盟（14个小组）申请STED超分辨率显微镜用于细胞生物学、胃肠病学、肾脏、免疫学和肿瘤学研究项目。在CBF有一个共焦显微镜，但是，没有STED显微镜的超分辨率荧光显微镜，一种技术，目前正在发展成为一个标准，这样的调查。未来的用户参与了许多DFG和BMBF的单一和联合研究项目，迫切需要STED显微镜。此次收购为这些第三方资助的项目提供了技术支持和方法上的进步。该显微镜将用于：亚细胞结构的超分辨率（超过阿贝极限）和高灵敏度成像，纳米结构蛋白质分布，多蛋白质复合物的组成。研究的结构包括紧密连接、极性、受体和信号复合物、脂滴、线粒体、病毒、纳米颗粒。为此，将进行固定样品的2D/3D成像和动态细胞过程的活细胞成像。与目前可用的共聚焦显微镜相比，目前应用的STED显微镜将实现相当高的空间分辨率（<在低水平的光毒性/光漂白下）。因此，它将允许高分辨率的共定位研究，这是我们的创新和高级别的结构和功能研究项目的成功处理的决定性因素。

The user consortium (14 groups) at the Campus Benjamin Franklin (CBF) of the Charité - Universitätsmedizin Berlin applies for a STED superresolution microscope for cell biological, gastroenterological, renal, immunological and oncological research projects. At the CBF there is one confocal micoscope, however, there is no STED microscope for superresolution fluorescence microscopy, a technique which is currently developing into a standard for such investigations. The future users are involved in numerous DFG and BMBF single and joint research projects, for which a STED microscope is urgently needed. The acquisition serves the technical support and methodological advancement of these third party funded projects. The microscope will be used for: superresolution (beyond the Abbe limit) and high-sensitivity imaging of subcellular structures, nanostructural protein distribution, composition of multi protein complexes. Structures investigated comprise tight junctions, polarity, receptor and signalling complexes, lipid droplets, mitochondria, viruses, nanoparticles. To this end 2D/3D-imaging of fixed samples and live cell imaging of dynamic cellular processes will be carried out. Compared to the currently available confocal microscope, the STED microscope presently applied for will enable considerably higher spatial resolution (< at low levels of photo-toxicity / photo-bleaching. Thus, it will allow high-resolution co-localization studies, which are decisive for the successful processing of our innovative and high-ranking structural and functional research projects.