Fluorescence Activated Cell Analyzer (FACS)

我代表德累斯顿理工大学的Medizinischen Fakultät Carl Gustav Carus请求支持购买一台分析仪，作为该学院核心设备的一部分。该核心设备位于该学院的中心研究大楼，具有两台分选器和一台分析仪。这些机器被大量用户广泛使用。该分析仪自2005年起投入使用，除因维修而延长停机时间外，一直被广泛使用。这台机器15年来的大量使用是相当了不起的，这在很大程度上要归功于专职技术人员提供的出色维护，以及各自公司明确的用户规则和定期服务。在过去的几年里，维修的需要增加了，但将是困难的，因为现在将再生产备件。2018年，机器有81天无法使用，2005年到2012年，每年只有13天发生这种情况。未来，通过在线预订的顺利使用也将由受过专门培训的技术人员来确保。来自18个或20个(2018/2019年)不同机构或研究小组的用户使用过这台机器，个人用户总数超过60人。在2013-2017年间，每月使用小时数超过190小时-分析仪已满负荷使用。由于维修增加，Usgae小时数在2018年降至每月约135小时，2019年降至每月147小时。有经验的用户也可以在周末和非工作时间使用这台机器。考虑到大量的使用和需求，停机时间对用户的影响是相当戏剧性的。购买更换机器的迫切需求是显而易见的。机器将被放置在相同的位置，不需要新的安装，性能参数将略有增加，即需要测量的参数从18个增加到25个。该分析仪对于教职员工的广泛研究活动是必不可少的，其中许多活动是基于第三方资金的。因此，我谨代表该设备的众多用户请求支持该应用程序，并给予其高优先级。教职员工的大型研究设备委员会给予了最高的优先考虑。

On behalf of the Medizinischen Fakultät Carl Gustav Carus of the Technischen Universität Dresden I am requesting the support of purchasing a analyzer as substitute for an older machine as part of the Core Facility of the faculty.This Core Facility is located in the central research building of the faculty and features two Sorters and one analyzer. These machines are extensively used by a large number of users. The analyzer is in operation since 2005 and is continuously and extensively used – except for increasing down times due to repair. The 15 years of heavy usage of this machine is quite remarkable and is largely due to excellent care provided by a dedicated technician and is due to clear user rules and regular service by the respective company. In the last few years, the need to repair increased, but will be difficult as now spare parts will be produced anymore. In 2018, the machine was non-functional at 81 days, in 2005- to 2012 this happened at only 13 days per year.The smooth usage via online booking will also be ensured in the future by the specially trained technician. Users from 18 or 20 (2018/2019) different institutions or research groups have used this machine with a total number of more than 60 individual users. The monthly hours of use were more than 190 in the years 2013-2017 – the analyzer is used to full capacity. As there were increased repairs, the usgae hours decreased to app.135 h/month in 2018 and 147 h/month in 2019. Experienced users are also allowed to use the machine on weekends and in off-hours. Considering the heavy use and demand, the consequences of down times are quite dramatic for the users.The urgent need to purchase a replacement machine is obvious. The machine will be placed in the same location with no new installations required, and will have a modest increase in performance parameters, i.e. 25 instead of 18 parameters to be measured. The analyzer is essentiell for a wide range of research activities, many based on third-party funds, of the faculty. Therefore I kindly request on behalf of the many users of this equipment to support this application and give it high priority. Highest priority has been given by the commission for large research equipment of the faculty.