Developing a novel high-throughput TCR-antigen pair identification method

在这几个月里，我培养了表达特定T细胞受体的T细胞，以及为T细胞呈递特定抗原的APCs（抗原呈递细胞）。我还培养了另一个APC它为T细胞提供了错误的配对抗原作为阴性对照。我尝试用显微镜在T细胞和apc的混合物中鉴定被识别的T细胞- apc对。通过成像，我成功地发现了一对T细胞与其特异性识别的APC相互作用。我也试过从溶液中提取t细胞- apc对。对于高通量扫描，我检查了当前显微镜的扫描速率，它每5分钟可以扫描10^5个细胞以上。在我取消这项资助之前，我的研究计划的其他步骤仍在进行中。

In these months, I have cultured the T cells which express a specific T cell receptor, and the APCs (Antigen presenting cell) which present a specific antigen for the T cell. And also I have cultured another APC which presents a wrong paired antigen for that T cell as a negative control.I have tried to identify the recognized T cell-APC pair in the mixture of T cells and APCs using a microscope. Using imaging, I have successfully found a pair of T cell interacted with its specific recognized APC. I also have tried to extract the T-cell-APC pairs from the solution.For the high-throughput scanning, I have checked the scanning rate of the current microscope, it can scan more than 10^5 cells per 5 mins.Until my cancellation of this grant, the other steps of my research plan are still ongoing.