Development of diagnostic tests for infectious diseases of mice and rats by microsphere fluorescent immunoassay

微球荧光免疫分析(MFI)允许使用少量血清同时检测多种啮齿动物病原体的抗体。在本研究中，我们检测了MFI在小鼠和大鼠感染的血清学诊断中的特异性和敏感性。用重组抗原和灭活抗原，用MFI检测小鼠和大鼠对9种主要病原体的抗体。MFI在不降低其特异性和敏感性的情况下，成功地在一次反应中对上述病原体抗体进行了多重检测。MFI非常适合于实验小鼠和大鼠的血清学诊断。

Microsphere fluorescent immunoassay (MFI) allows simultaneous detection of antibodies to multiple rodent pathogens using a small volume of sera. In the present study, we examined the specificity and sensitivity of MFI in serologic diagnosis for infections on mice and rats. Antibodies to major 9 pathogens in mice and rats were measured by MFI using recombinant and inactivated antigens. MFI succeeded in multiplex detection of antibodies to the above-mentioned pathogens in a single reaction well without decreasing its specificity and sensitivity. MFI is highly suitable for serodiagnosis in laboratory mice and rats.