Analytical 5-laser multi-parameter flow cytometer

在单细胞水平上对异质样品中的细胞进行多参数分析是理解健康和疾病背景下的生物学关系和机制的最重要技术之一。关键技术是使用流式细胞术或质谱细胞术分析悬浮液中的单细胞，以及使用组织病理学或成像质谱细胞术分析组织中的细胞。流式细胞术能够使用多种参数以高通量分析细胞。对用于细胞分析和细胞分选的高度专业化设备的高需求以及高成本主张集中提供此类技术。集成到中央核心设备中，可以有效地使用仪器，并为用户提供专家支持。中央流式细胞仪和质谱仪核心设备'Charité| 10多年来，BIH Cytometry Core一直在Charité CVK工厂为多参数单细胞分析和细胞分选提供专家服务和最新设备。然而，目前在Charité米特地区没有集中使用流式细胞仪的设施，而在Charité米特地区参与这项申请的工作组则依赖于使用此处所要求的设备进行其跨学科研究项目。到目前为止，其他研究所的设备可以用于一些项目，或者分析可以分解为几种参数较少的方法。其他研究所的设备已无法满足需要。由于样本材料的限制，越来越需要同时分析尽可能多的参数。因此，Charité的中央流式细胞仪和质谱细胞仪核心设施在此申请购买分析型5激光多参数流式细胞仪。与5激光高速细胞分选设备一起，该仪器应构成扩展中央核心设施的基础，也超出了此处列出的申请人群体。将该仪器纳入当时的跨地点中央核心设施，置于一个共同的管理结构之下，应能确保该技术的迅速可用性。已建立的质量管理结构被接管，通过经过验证的培训概念来保证用户的培训。

The multi-parameter analysis of cells within a heterogeneous sample at the single cell level is one of the most important technology for understanding of biological relationships and mechanisms in the context of health and disease. Key technologies are the analysis of single cells in suspension using flow cytometry or mass cytometry, as well as the analysis of cells in the tissue context using histopathology or imaging mass cytometry. Flow cytometry enables cells to be analyzed using a variety of parameters at high throughput. The high demand for highly specialized devices for both cell analysis and cell sorting and the high costs argue for a centralized provision of such technologies. The integration into a central core facility allows efficient use of the instruments and expert support for the users.The central Flow & Mass Cytometry Core Facility 'Charité | BIH Cytometry Core ' has been offering expert service and the latest equipment for multi-parameter single cell analysis and cell sorting at the Charité CVK site for more than 10 years. However, there is currently no centralized access to flow cytometry devices at the Charité Mitte site.The working groups at the Charité Mitte location involved in this application are dependent on the use of the equipment requested here for their interdisciplinary research projects. So far, devices from other institutes could be used for some of the projects or analyzes could be broken down into several approaches with fewer parameters. The devices of the other institutes are no longer available to the extent necessary. The limitation of the sample material increasingly requires a simultaneous analysis of as many parameters as possible.The Charité's central Flow & Mass Cytometry Core Facility therefore applies here for the purchase of an analytical 5-laser multi-parameter flow cytometer. Together with a 5-laser high-speed cell sorting device, this instrument should form the basis for the expansion of the central core facility also beyond the group of the applicants listed here. The integration of the instrument into the then cross-location central core facility under a common management structure should ensure the rapid availability of the technology. Established quality management structures are taken over and the training of the users is guaranteed through proven training concepts.