Identification of the regulatory factor for insect-form transformation of trypanosomes from tsetse flies

为了控制非洲昏睡病，这是由锥虫寄生虫（锥虫属）感染。在哺乳动物血液中，需要了解细胞在感染时分化为昆虫形式寄生虫的调节机制。本研究利用RACE PCR技术克隆了Glossina丝氨酸蛋白酶1（Gsp1）的全长基因，并对其进行了序列分析，结果表明Gsp1具有胰蛋白酶样丝氨酸蛋白酶结构域。结合前人的研究，其中一个丝氨酸蛋白酶基因的复制可能获得控制锥虫型转化的新功能。我

To control the African Sleeping Sickness, which is caused by the infection of trypanosome parasites (Trypanosoma spp.) into mammalian blood, it is required to understand the regulatory mechanisms for cell differentiation into insect form parasite at the timing of infection. In this study, the full length of Glossina serine protease 1 (Gsp1) was isolated by RACE PCR as the candidate of regulatory molecules, and it was showed that Gsp1 have the trypsin-like serine protease domain. Considering the previous studies, one of the serine protease genes duplicated might gain the novel function for controlling the insect-form transformation of Trypanosoma. I