Regeneration of invertebral disc by iPS cells and challenge for in situ iPS cell bank.

椎间盘细胞与iPS细胞分化的稳定方法尚未建立。本实验采用iPS细胞与IVD细胞共培养的方法分化iPS细胞。首先，我们直接将iPS细胞与IVD细胞共培养。然而，iPS细胞分化成纤维细胞样细胞，不具有IVD细胞的特征。然后将iPS细胞诱导成胚体，并将胚体细胞用维甲酸处理7 d。处理后，间充质干细胞样细胞分化。将分化后的细胞与成骨细胞诱导剂共培养3周。茜素红染色证实了间充质干细胞样细胞分化成骨细胞的潜力。

The stable method to differentiate intervertebral disc (IVD) cell from iPS cells has not been established yet. Here we employed the co-culture method with iPS cells and IVD cells to differentiate iPS cells. At first, we directly co-cultured iPS cells with IVD cells. However iPS cells differentiated fibroblast like cells, not have the characteristics of IVD cells. Next we induced embryoid body from iPS cells, in addition embryoid body cells were treated with retinoic acid for seven days. After treatment, mesenchymal stem like cells were differentiated. Furthermore these differentiated cells were co-cultured with osteoblast inducer reagent for three weeks. We have confirmed the potential of mesenchymal stem like cells to differentiate the osteoblast cell by Alizarin red staining.