Epithelium-intrinsic microRNAs contribute to the mucosal immune homeostasis through ensuring epithelial cell differentiation

为了阐明microRNA(MiRNA)在肠上皮细胞分化和动态平衡中的作用机制，我们对杯状细胞分化的结肠癌细胞株HT29-MTX和正常小鼠的肠上皮细胞以及Dicer1(DICER-KO)小鼠肠上皮细胞特异性缺失进行了基因芯片分析。使用Inenuity Path分析软件和miRNA靶标预测数据库对数据集进行分析。我们发现了几个可能参与杯状细胞或M细胞分化的microRNAs。此外，对DICER-KO小鼠的表型分析表明，这些小鼠可能是有用的溃疡性结肠炎模型。

To elucidate the mechanisms of intestinal epithelial cell differentiation and homeostasis by microRNA(miRNA), we performed microarray analysis of the goblet cell differentiated colon cancer cell line HT29-MTX and intestinal epithelium from control mice or intestinal epithelial cell specific deletion of Dicer1(Dicer-KO) mice. The data sets were analyzed by Ingenuity Pathway Analysis software and miRNA target prediction database. We found several microRNAs that might be involved in goblet cell or M cell differentiation. In addition, phenotype analysis of Dicer-KO mice indicated that these mice might be useful as an ulcerative colitis model.