Hybrid formation and improvement for bonding to tooth hard tissue

基于粘附性表面处理和功能大分子的粘结剂的改进使粘接性牙科技术在从防龋术到修复术的广泛领域得到了应用。然而，在严重的口腔内条件下的粘连在边缘密封性、粘接强度和耐用性方面存在问题。主要原因可能是牙齿结构和粘结剂(树脂)之间的界面层的形成、结构和机械性能。为了获得良好的界面层形成，本研究开发并分析了一种界面层结构元素磷灰石和胶原之间具有较强相互作用的底漆的性能。1)通过合成氨基酸甲基丙烯酸酯(NMGly、NMBU、NAASP、NMVa、NMCA等)，并在一系列浓度的蒸馏水中溶解，制备了底漆。2)通过…测定，确定了底物与牙本质胶原之间的相互作用更多的松弛时间(T1)与NMR.3)为了测量粘结强度，用碳化硅纸(600-GRIT)研磨的人和牛牙本质用实验底漆处理，然后应用商用的酯-磷酸粘结剂，并用复合树脂填充。4)用扫描电子显微镜和透射电子显微镜对粘接界面和粘接表面进行了结构分析，不同氨基酸类型的底漆与胶原蛋白的相互作用不同。强组合顺序为：Va&Gt；Bu&Gt；Ca&Gt；Ala&Gt；Gly。40%磷酸处理后的粘结剂与牙本质的粘结强度为9~16 Mpa，与胶原蛋白和底漆相互作用的结果呈正相关。在粘接面的牙本质侧形成4~6μm宽的胶原和聚合树脂杂合层。研究结果表明，一种与附着底物有较强相互作用的底漆是粘合必不可少的。较少

Improvements in adhesives based on adherent surface treatment and functional macromolecules have permitted the use of adhesive dentistry technique in a wide field, from caries prevention through prosthetic rehabilitation. However, the adhesion within the severe intra-oral conditions presents problems in marginal sealing, adhesive strength and durability. The main reasons may be the formation, structure and mechanical properties of the interfacial layer between the tooth structure and the adhesive (resin). Aiming to obtain a sound interfacial layer formation, in this study we developed and analyzed the properties of a primer with strong interaction between the structural elements of the interfacial layer, which are apatite and collagen.1) The primer was prepared by synthesis of aminoacid methacrylates (NMGly, NMBu, NAAsp, NMVa, NMCa, etc) and dissolution in distilled water in a series of concentrations. 2) The interaction between primer and dentinal collagen was obtained by measurement … More of the relaxation time (T1) with NMR.3) For bond strength measurements, human and bovine dentin ground with silicon carbide paper (600-grit) were treated with the experimental primers, followed by application of a commercially available ester-phosphate based bonding agent and filling with composite resin. 4) The SEM and TEM were used for structural analysis of the adhesive interfaces and adherent surfaces.The interaction between collagen and primers based on amino-acid methacrylate solutions varied depending on the type of aminoacid. The sequence of strong combinations was as follows : Va>Bu>Ca>Ala>Gly. The bond strength of bonding agents after treatment to dentin with 40% phosphoric acid and each type of primer was from 9 to 16 Mpa, showing correlation with the results for collagen and primer interactions. A hybrid layer of collagen and polymerized resin 4 to 6 μm wide was formed in the dentin side of the adhesive interface. The results of this study show that a primer with strong interaction to the adherent substrate is indispensable for adhesion. Less