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Control of interface between tooth tissue and resin by amino acid derivative

氨基酸衍生物控制牙齿组织和树脂之间的界面

基本信息

批准号：
13470412
负责人：
SUZUKI Kazuomi
金额：
$ 10.37万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470412/
关键词：
Tooth Adhesion Composite resin Surface treatment Collagen Apatite Hybrid Functional monomer

项目摘要

The bonding technique to tooth tissue including etching procedure and adhesive application was developed to get functional reconstruction of the caries in a wide area application. However, it is difficult to make a perfect bonding, a good characteristic of marginal gap closure, bonding strength and durability under a severe condition of buccal cavity. The main problem is on the formation and the physical properties of the interface layer between tooth tissue and resin. Therefore, we synthesize the amino acid derivative (NMωA) which has chemical compound affinity, especially by examining the interaction with dentinal collagen. In this study, in order to specify the NMωA formed by the interaction of collagen functional group, the relaxation time of NMωA in the collagen model chemical, compound that has an end part of imino unit and carboxyl unit was measured.The measurement of ^<13>CNMR (T_1) is as follows. Oligopeptide of 10% (5 bodies of prolylprolylglycine, unit of protein/amino acid) … More were dissolved in hydrochloric acid added deuterium oxide aqueous solution. The aqueous solution was manufactured at the pH of 1.7. After homogenized, 0.6g of this oligopeptide aqueous solution was placed in the NMR sample tube. 6.73×105 or 20.19 x 10^5 mol of NMωA was used as the NMR sample. T_1 was measured using an EX270MHz spectrometer (Nihon Denshi), which is performed with 180゜-τ-90゜ pulse sequence, in the temperature of 25℃.As oligopeptides were coexist in NMωA aqueous solution, in order to examine an interaction of amide unit and carbonyl unit in the molecule of NMωA with the oligopeptide, the value of T_1 of carbonyl carbon which revert to these functional groups was similar to that was coexists in the deashing dentine. Compared to that of the non-coexistence chain, there was a great decrease. From the above result, the presents of amide and carboxyl unit in the molecule of NMωA showed that the side chains functional group of carbonyl unit and hydrogen bond in the collagen are formed. Furthermore, in case of hydroxymethacrylate (HEMA), it was identified that there was a strong interaction between the carbonyl unit in the same peptide and the carbonyl unit in the molecule. Less

本研究发展了一种包括酸蚀和粘接剂在内的牙体组织粘接技术，以实现龋损的功能性修复，并得到了广泛的应用。然而，在口腔条件恶劣的情况下，很难获得理想的粘接效果，难以达到边缘间隙闭合、粘接强度和持久性的良好特性。目前存在的主要问题是牙体组织与树脂界面层的形成及其物理性质。因此，我们合成了具有化合物亲和性的氨基酸衍生物（NMωA），特别是通过研究与牙本质胶原的相互作用。在本研究中，为了确定由胶原官能团相互作用形成的NMωA，测量了具有亚氨基单元和羧基单元的末端部分的胶原模型化合物中的NMωA的弛豫时间<13>。10%的寡肽（5个脯氨酰脯氨酰甘氨酸体，蛋白质/氨基酸单位） ...更多信息溶于盐酸中，加入氧化氘水溶液。在pH 1.7下制备水溶液。均质化后，将0.6g该寡肽水溶液置于NMR样品管中。使用6.73×105或20.19 × 10^5 mol NMωA作为NMR样品。T_1用EX 270 MHz谱仪测量（Nihon Denshi），在25℃下，用180 <$-τ-90 <$脉冲序列进行。由于寡肽共存于NMωA水溶液中，为了检查NMωA分子中的酰胺单元和羰基单元与寡肽的相互作用，还原成这些官能团的羰基碳的T_1值与脱灰牙本质中共存的羰基碳的T_1值相近。与非共存链相比，有很大的下降。从以上结果可以看出，NMωA分子中酰胺基和羧基的存在，说明胶原中形成了羰基侧链官能团和氢键。此外，在羟基甲基丙烯酸酯（HEMA）的情况下，确定在相同肽中的羰基单元与分子中的羰基单元之间存在强相互作用。少