STUDIES ON THE IMPROVEMENT OF PRODUCTION EFFICIENCY OF POULTRY USING ANTISENSE NUCLEOTIDES

本实验研究了IGFBP-2反义寡核苷酸（ODN）对鸡组织IGFBP-2基因表达的影响。将反义IGFBP-2 ODN直接注射入肝或脑室。对照鸡注射溶媒。注射反义ODN后2 h，肝脏IGFBP-2基因表达量下降至对照组的30%左右，脑组织IGFBP-2 mRNA表达量在注射后2 h无明显变化，注射后6 h下降至对照组的60%左右。这些结果表明，应用反义ODN成功地抑制了肝脏和脑中IGFBP-2基因的表达，并且肝脏IGFBP-2基因表达被反义ODN抑制的速度比脑快。结果表明，肌生成抑制素反义寡脱氧核苷酸（myostatin oligodeoxynucleotide，ODN）对鸡胚成肌细胞蛋白质合成有明显的抑制作用。观察到不同胎牛血清浓度和转染处理之间的显著相互作用。阳离子脂质体不影响成肌细胞蛋白质合成。无论培养液中胎牛血清浓度如何，反义myostatin ODN均可显著降低鸡胚成肌细胞的蛋白质合成。这些结果表明，减少肌肉生长抑制素减少蛋白质合成的鸡胚成肌细胞和肌肉生长抑制素对蛋白质合成的影响将是独立的IGF-I或IGF-II。

We have examined the influence of antisense oligo deoxynucleotide (ODN) of IGFBP-2 on tissue IGFBP-2 gene expression in chicks. Antisense IGFBP-2 ODN was directly injected into the liver or cerebroventricle. Control birds were injected with vehicle. The hepatic IGFBP-2 gene expression was decreased to approximately 30% of the control at 2 h after injection of antisense ODN.In the brain of chickens injected with antisense ODN, IGFBP-2 mRNA level did not change after 2 h of injection and decreased to approximately 60% of the control after 6 h of injection. These results showed that the expression of IGFBP-2 gene in the liver and brain was successfully suppressed by administrating antisense ODN and that hepatic IGFBP-2 gene expression was quickly suppressed by antisense ODN compared with the brain.The interactive effect between varying fetal calf serum concentrations (1% and 10% in the medium) and transfection treatment with antisense and sense myostatin oligodeoxynucleotide (ODN) on protein synthesis of chicken embryo myoblasts was examined. The significant interaction between varying fetal calf serum concentrations and transfection treatment was observed. The cationic liposome did not affect myoblast protein synthesis. Protein synthesis of chicken embryo myoblasts was significantly decreased by antisense myostatin ODN regardless of varying fetal calf serum concentrations in the medium. These results suggest that a decrease in myostatin reduced protein synthesis of chicken embryo myoblasts and that the influence of myostatin on protein synthesis would be independent of either IGF-I or IGF-II.