Organ Transplantation from non-heart beating

1.在前人实验的基础上，对移植后不能存活的热缺血90min的胰腺移植物，采用二层法保存5h即可成功移植。在两层保存过程中蛋白质合成与移植物复苏有关的假设下，进行了以下实验。用两层法(全氟化学/UW液)保存热缺血90min的犬胰腺，保存时间为20℃，保存时间为5小时。在此保存液中分别加入氚胸苷、氚尿苷和氚亮氨酸，以定量测定移植物内DNA、RNA和蛋白质的合成。根据颈部皮下腔自体移植后移植物存活率来判断移植物的存活率。7例(0%)移植物在热缺血后立即移植时无一存活，而在20℃两层保存5h后移植的7例移植物全部存活(100%)。在两层法保存期间，RNA和随后的蛋白质合成显著增加，而不是UW简单冷藏。相反，DNA合成并没有跟随RNA和蛋白质的合成。结论：1.蛋白质合成可能与移植胰腺在两层法亚低温保存过程中缺血后细胞的恢复过程有关。一些报道表明，热休克蛋白(HSPs)在细胞恢复中具有重要作用。我们用双层法研究了热休克蛋白在减轻缺血再灌注损伤中的作用。免疫组织化学和免疫印迹结果显示，HSP-60可能与热缺血损伤的胰腺功能恢复有关。

1. Based on our previous canine experiments, pancreas grafts subjected to 90 min warm ischemia, which cannot survive after implantation with no treatment, could be successfully transplanted after 5-hour preservation by the two-layer method. On the hypothesis that protein synthesis during the two-layer preservation would be associated with this graft resuscitation, the following experiments were undertaken. We preserved canine pancreases damaged by 90-min warm ischemia using the two-layer method (perfluorochemical/UW solution) for 5 hrs at 20℃. Intragraft DNA, RNA and protein synthesis were quantitated durins preservation by determining incorporation of tritiated thymidine, tritiated uridine and tritiated leucine, respctively, added in this preservation solution. Graft viability was judged from graft survival after autotransplantation in the cervical subcutaneous cavity. None of 7 grafts (0%) survived when they were immediately transplanted after warm ischemia, while all of 7 grafts (100%) transplanted after the two-layer preservation for 5 hrs at 20℃ survived. Significant increases in RNA and subsequent protein syntheses were observed during preservation by the two-layer method, not by UW simple cold storage. In contrast, DNA synthesis did not follow RNA and protein synthesis. We concluded that protein synthesis might be associated with the process of postischemic cellular recovery of the pancreas graft during mild hypothermic preservation by the two-layer method.2. Some reports have indicated that heat shock proteins (HSPs) have important functions in cellular recovery. We studied the role of HSPs in reducing ischemia-reperfusion injury during preservation by the two-layer method. From the results of immunohistochemical examinations and western-blotting of HSPs, HSP-60 was likely associated with functional recovery of warm ischemically damaged pancreas by the two-layer method.