A Study on Designed Hapten Aimed at Anti-ciguatoxin Monoclonal Antibody

合成了雪卡毒素abc环片段为C2外聚物（1:1）。该混合物被设计为制备抗雪卡毒素单克隆抗体的可能半抗原，并与载体蛋白（KLH）偶联，然后用于小鼠免疫。获得的三种单克隆抗体（mab）对半抗原表现出明显的反应性。每个单抗都区分了abc环片段的C2羟基的构型。对2s片段有反应的单抗与雪卡毒素交叉反应。此外，还开发了新的高效的烯烃合成方法，制备了几种设计的半抗原，并与KLH和BSA偶联，用于小鼠免疫。

The ABC-ring fragment of ciguatoxin was synthesized as a C2 epimeric mixture (1 : 1 ). The mixture, which was designed as a possible hapten for preparing an anti-ciguatoxin monoclonal antibody, was conjugated with a carrier protein (KLH) and then used for immunization of mice. The three monoclonal antibodies (mAbs) obtained have shown an appreciable reactivity to the hapten. Each mAb discriminated configuration of the C2 hydroxy group of the ABC-ring fragment. The mAb which showed reactivity to the 2S-fragment was cross-reacted with ciguatoxin. Furthermore, new and efficient synthetic methods utilizing olefin methathesis were developed and several designed haptens have been prepared and conjugated with KLH and BSA, which have been used for immunization of mice.