Functional change of exudative polymorphonuclear neutrophil in surgical stress.

我们研究了手术应激时营养不良对中性粒细胞（PMN）介导的免疫功能的影响。获得的结果如下：将雄性C57 BL/6 J小鼠（n=204）分配到自由采食、中度和重度饮食限制组，分别接受自由采食（132 g/kg/天）、66和33 g/kg/天的小鼠食物7天。在有/没有一天再喂养的饮食限制后，向小鼠施用糖原i.p.to诱导细胞渗出。结果：（1）限食7 d后，循环PMN表面CD 11b/CD 18、CD 62 L表达明显降低;（2）限食7 d后，循环PMN表面CD 11b/CD 18、CD 62 L表达明显降低; 2）在腹膜炎早期，7天的饮食限制减少了随后的PMN渗出到腹膜腔中。3）循环PMN上的CD 11b和CD 18表达，7天的饮食限制也损害了吞噬功能5）7天的饮食限制上调了渗出性PMN的ROI产生6）7天的饮食限制降低了PLF中MIP-2的水平。

We investigated thc influence of malnutrition on neutrophil Polymorphonuclear neutrophil (PMN)-mediated immunity in surgical stress. Obtained results are as follows : Male C57BL/6J mice (n=204) were assigned to ad libitum, moderate and severe dietrestricted groups receiving mouse chow ad libitum (132 g/kg per day), 66 and 33 g/kg per day for seven days, respectively. After dietary restriction with/without one day of refeeding, mice were administered glycogen i.p.to induce cell exudation. CD11b CD18 and CD62L expressions on circulating PMNs, phagocytosis and ROI production by exudative PMNs, were measured after glycogen installation.The levels of PMN specific chemokine, macrophage inflammatory protein (MIP)-2 in PLF were also measured.1)Seven days of dietary restriction decreased CD11b/CD18 expression on circulating PMNs,2) Seven days of dietary restriction decreased subsequent PMN exudation into the peritoneal cavity early in peritonitis.3)Both CD11b and CD18 expression on circulating PMNs, correlated significantly with numbers of exudative.4) Seven days of dietary restriction also impaired phagocytosis5) Seven days of dietary restriction upregulated ROI production by exudative PMNs.6)Seven days of dietary restriction decreased MIP-2 levels in PLF.