An experimental study about anti-local inflammation effects of liquid ventilation.

本研究的目的是评价部分液体通气（PLV）对LPS诱导的大鼠急性肺损伤模型BAL中TNF-_a、MIP-2、cinc1浓度的影响，以及CD11b和巨噬细胞亚群表面表达的影响。本研究设计为实验性、前瞻性、对照研究。34只大鼠被分为三组。30分钟组气管内LPS给药后进行气体通气30分钟；GV组在LPS给药后给予气体通气30min，再持续气体通气2h；LPS给药后给予PLV组30min气体通气，再用全氟隆进行PLV治疗2h。在每次通气结束时，对动物实施安乐死并进行BAL。ELISA法检测BAL上清中TNF α、MIP-2、cinc1的浓度。GV组与PLV组在TNF α （GV: 18828.7±10676.6,PLV: 20008.1±1573.8，代表平均值±SEM）、MIP-2（13141.2±943.6,PLV: 14433.5±147.0）、cnc -1 （GV: 8765.2±1129.1,PLV: 10144.4±1220.7）方面无显著差异。用流式细胞术检测PLV对BAL细胞中性粒细胞表面CD11b表达和抗巨噬细胞亚群表达的影响。CD11b阳性表达比（阳性细胞/获得细胞）（GV: 63.6±8.4,PLV: 60.5±5.4）差异无统计学意义。巨噬细胞表达率显著升高（GV: 5.6±1.5,PLV: 14.0±1.3,p<0.005）。在LPS诱导的急性肺损伤模型中，全氟化铅对BAL上清TNF α、MIP-2、cnc -1浓度及CD11b细胞表面表达均无显著影响。然而，每单位巨噬细胞在BAL细胞中的比例增加了PLV。

The objectives of this study is to evaluate the effect of partial liquid ventilation (PLV) with respect to TNF-_a, MIP-2, CINC-1 concentration in BAL in LPS induced acute lung injury model in rats, and the surface expression of CD11b and macrophage subset as well. This study was designed as experimental, prospective, controlled study. Thirty-four rats assigned to three groups. The 30 minutes group was treated with gas ventilation for 30 minutes after intratracheal LPS administration ; the GV group was treated with 30 minutes of gas ventilation after LPS administration, then gas ventilation was continued for following 2 hours ; and the PLV group was treated with 30 minutes of gas ventilation after LPS administration, then PLV with Perflubron was performed for following 2 hours. At the end of each ventilations, animals were euthanized and BAL was performed. The concentrations of TNF α, MIP-2, CINC-1 were measured in BAL supernatant by ELISA. There were no significant difference between GV and PLV group in TNF α (GV : 18828.7 ± 10676.6, PLV : 20008.1 ± 1573.8 which represents mean ± SEM), MIP-2 (13141.2 ± 943.6, PLV : 14433.5 ± 147.0), and CINC-1 (GV : 8765.2 ± 1129.1, PLV : 10144.4 ± 1220.7). The effects of PLV on cell surface e pression of CD11b in neutrophils, and that of anti macrophage subset were determined by the flow cytometric analysis in BAL cells. There was no significant difference of positive expression ratio (positive cells / aqurired cells) in CD11b (GV : 63.6 ± 8.4, PLV : 60.5 ± 5.4). However macrophage expression ratio was significantly increased (GV : 5.6 ± 1.5, PLV : 14.0 ± 1.3, p<0.005). BAL supernatant TNF α, MIP-2, CINC-1 concentration is not affected significantly by PLV with perflubron in this rodent model of LPS induced acute lung injury, nor CD11b cell surface expression. However the ratio of macrophage per unit in BAL cells was increased by PLV.