Molecular manipulation and application of thermophilic cyanobacteria

建立了嗜热蓝细菌细长热聚球藻BP-1的转化感受态克隆。与S合作。Tabata（Kazusa DNA研究所），我们确定了完整的核苷酸序列，从T. elongatus中表达，并在E.从T. coli中分离得到藻蓝蛋白。细长且结晶。在T中构建了psbI、psbT、psbV和psbK的基因破坏突变体。细长的从这些突变体中分离出光系统II复合物，构建了一系列针对T.从野生型长形木霉中分离出光系统II复合物，并对其进行功能分析。火山和结晶。的3d

A transformation-competent clone of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1 was established. In collaboration with S. Tabata (Kazusa DNA Res. Inst.), we determined the complete nucleotide sequence.A gene for a novel flavin-binding protein was cloned from T. elongatus and expressed in E. coli.Phycocyanin was isolated from T. elongatus and crystalled. X-ray diffraction data at 2.0 A resolution was obtained.Gene disruption mutants were created for psbI, psbT, psbV and psbK in T. elongatus. Photosystem II complexes were isolated from these mutants.A number of site-directed mutants were constructed for the extrinsic 33 kDa protein of T. elongatus and were analyzed functionally.O_2-evolving Photosystem II complex was isolated from wild type of T. vulcanus and crystallized. The 3D