Autotrophic carbon dioxide fixation reaction within non-Calvin type pathway

我成功地在体外证明了丙酮酸：铁氧还蛋白氧化还原酶和2-氧葡萄糖酸：铁氧还蛋白氧化还原酶的二氧化碳固定反应，它们是在嗜热氢杆菌中作为还原性TCA循环的关键酶。两种酶都是可逆的。因此，在使用一种酶进行实验时，使用另一种酶进行还原铁氧还蛋白的再生。同时，对异柠檬酸脱氢酶反应进行了详细的分析，表明生物素化蛋白在二氧化碳固定反应中是必不可少的。酰基辅酶a羧化酶也从一种嗜热的硫氧化太古菌（Acidianus brierieyi）中纯化并鉴定。纯化后的酶与另一种乙酰辅酶a羧化酶具有相同的特性。尽管如此，它与另一种乙酰辅酶a羧化酶有几个特征上的不同。

I succeeded in demonstrating in vitro carbon dioxide fixation reactions of both Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase, which are operative within Hydrogenobactei-thermophilus as key enzymes of reductive TCA cycle. Both enzymes are reversible. Therefore, during the experiment of one enzyme, the other enzyme was used for the regeneration of reduced ferredoxin. Also, isocitrate dehydrogenase reaction was analyzed in detail, biotinylated protein was shown to be essential for the carbon dioxide fixation reaction.Acyl-CoA carboxylase was also purified and characterized from a thermophilic sulfur oxidizing archeon, Acidianus brierieyi, which was a first example from archaea. The purified enzyme shared common characteristics with another acetyl-CoA carboxylases. Still, it had several characteristic differences from another acetyl-CoA carboxylases.