Studies on eukaryotic inorganic carbon transporters based on the gene network involved in photosynthetic acclimation

衣藻通过感知CO_2的有效性和光照来调节光合作用特性以适应CO_2限制胁迫，这是通过诱导一组碳浓缩机制的基因来实现的。我们分析了编码碳酸酐酶的基因Cah1的调控，以阐明CO_2信号转导途径。利用Ars-Report与Cah1启动子融合，阐明了参与CO_2调节的顺式作用元件和DNA结合蛋白(Kucho等人)。植物生理素。2003年，Yoshioka等人。植物细胞，2004)。对调控突变体基因表达的全基因组分析表明，CO_2响应基因被分成几类(Miura Factory Physiol.，2004)。尽管主调节器CCM1的功能(Fukuzawa等人PNAS 2001)，一些编码叶绿体被膜蛋白和阴离子转运蛋白的低CO_2诱导基因是真核藻Chlamydomonas rehardtii无机碳转运体的良好候选基因。

Chlamydomonas modulates photosynthetic characteristics to acclimate to CO_2-limiting stress by sensing CO_2 availability and light by inducing a set of genes for carbon-concentrating mechanism. We have analyzed regulation of a gene Cah1 encoding a carbonic anhydrase to elucidate the CO_2-signal transduction pathways. By using Ars-reporter fused with Cah1 promoter, cis-acting elements and DNA-binding proteins involved in CO_2-regulation were elucidated (Kucho et al. Plant Physiol. 2003, Yoshioka et al. Plant Cell, 2004). Genome wide analysis of gene expression of regulatory mutants revealed that CO_2-responsive genes were grouped into several categories (Miura Plant Physiol., 2004). Although functions of a master regulator CCM1 (Fukuzawa et al. PNAS 2001) is not known, several low-CO_2 inducible genes encoding chloroplast envelop proteins and anion transporters were good candidates for inorganic carbon transporters in eukaryotic algae, Chlamydomonas reinhardtii.