Development of on-demand multifunctional delivery systems using dendron lipid assemblies

我们开发了各种类型的功能性树突轴承脂质，其中包括基因递送树突脂质，温度敏感的树突脂质，pH敏感的树突脂质，和MR成像树突脂质。我们发现，基因递送树枝状脂质通过疏水相互作用和质子海绵效应实现了对培养细胞的高效基因转染。温度树枝状脂质被证明提供了一种新型的温度响应性囊泡，在特定的温度下进行形态转变。pH敏感的树枝状脂质形成高度pH响应囊泡与蛋黄磷脂酰胆碱释放内容物的细胞内体。树突状脂质的MR成像可用于检测脂质体的生物分布和积聚，从而导致个体化化疗。树突脂质技术有望为开发新的有效的非侵入性癌症治疗方法做出贡献。

We developed various types of functional dendron-bearing lipids, which include gene delivery dendron lipids, temperature-sensitive dendron lipids, pH-sensitive dendron lipids, and MR imaging dendron lipids. We found that gene delivery dendron lipids achieved highly efficient gene transfection into culture cells through hydrophobic interaction and proton sponge effect. Temperature-dendron lipids were shown to provide a new type of temperature-responsive vesicles that undergo morphology transition at a specific temperature. pH-Sensitive dendron lipids formed highly pH-responsive vesicles with egg yolk phosphatidylcholine that release contents in the endosome of a cell. MR imaging dendron lipids were useful for detection of bio-distribution and accumulation of liposomes, which can lead to personalized chemotherapy. The dendron lipid technology is expected to contribute development of new efficient noninvasive cancer therapy.