denitrifying factory

迫切需要对地下水进行适当管理，以应对日益严重的缺水威胁。硝酸盐污染是地下水的主要问题。为减少地下水硝酸盐污染，采用稳定同位素示踪法对地下水硝酸盐浓度和反硝化活性进行了原位测定。利用qPCR技术对反硝化功能基因进行基因分析，揭示了相应的微生物种群。反硝化的限制也阐明。乙酸盐显示了最高浓度的测得的有机化合物溶解在地下水中。而加入乙酸盐的15 NO3-培养地下水的脱氮活性比原位培养提高了约2倍。威尔斯周围的土壤微生物使反硝化作用增强了约三倍。从而为构建地下水原位高效脱氮系统提供了基本认识。

Adequate management of groundwater is strongly required against increasing threat of water shortage. Nitrate contamination is remained problems for groundwater. To minimize nitrate contamination we surveyed nitrate concentration and estimated in situ denitrification activity of groundwater by stable isotope tracer experiment. And we revealed corresponding microbial populations using gene analysis with qPCR targeting denitrification functional genes. Constraints of denitrification are also elucidated. Acetate showed the highest concentration among the measured organic compounds dissolved in the groundwater. And when the groundwater was incubated with 15NO3- by adding acetate debitirification activity increased about twice compared with the incubation under the in situ condition. And soil microbes around the wells enhanced denitrification about three times. Basic understandings were thus obtained to construct in situ highly active denitrification system for the groundwater.