Live-cell Imaging Super-resolution Microscope System

在这里，我们要求一种基于STED（受激发射耗尽）技术的超分辨率显微镜系统，该系统将主要用于活细胞STED应用。因此，该系统应提供STED成像模式，以减少耗尽激光在样品上的能量，同时在x， y和z方向上保持高光学分辨率。由于STED不适用于植物组织，该系统应提供另一种选择，以显着提高植物样品的分辨率。该系统将集成在成像核心设施的多用户环境中。因此，系统的处理应该是用户友好的，系统应该提供标准的共聚焦功能旁边的超分辨率选项。

Here, we request a super-resolution microscope system based on STED (stimulated emission depletion) technique, which will be mainly used for live-cell STED applications. Therefore, the system should provide STED imaging modalities to reduce the amount of energy of the depletion lasers on the sample, while preserving high optical resolution in x, y and z direction. Since STED is not applicable to plant tissue, the system should provide an alternative option to significantly increase resolution in plant samples. The system will be integrated in the multi-user environment of an imaging core facility. Therefore, the handling of the system should be user-friendly, and the system should offer standard confocal functionality next to the super-resolution options.