New approach for drug monitoring by using human sweat secreted

人体汗液是体液的一种。人体汗液分泌在皮肤表面，因此很容易非侵入性地收集。我们打算用方便的采样方法收集人体汗液，并将其用于药物监测。首先，我们开发了一种简便的汗液采样方法，并同时测量了汗量。简便的取样方法是用一个装有少量乙醇-水溶液的小瓶子(内部体积为0.6ml)存档。贴在人体皮肤表面的收集汗液的小瓶，时间为3到5分钟。采用高效液相色谱仪、毛细管电泳法、GC/MS联用仪等手段对大鼠汗液中的咖啡因成分进行分析。1.测定服用含咖啡因片剂后大鼠汗液中咖啡因的含量变化。在汗液中发现咖啡因及其代谢物，其浓度随时间的不同而不同。服药后也发现可待因。在汗液中发现了有机羧酸和葡萄糖，并对其摄入后的浓度变化进行了研究。挥发性有机气体直接从皮肤表面收集。气体被引入冷集30秒，然后切换到气相色谱分析。研究了新型诱捕装置，并申请了国际专利。通过以上研究，我们得出结论，利用汗液进行药物监测是可能的，因为汗液中含有大量与血液中的浓度有一定关系的成分。汗水的使用非常有吸引力，因为它很容易被人们用来测试身体状况，以便他们控制日常生活。

Human sweat is one of the body fluids. Human sweat is secreted on a skin surface, so that it is easy to collect non-invasively. We intend to collect human sweat by handy sampling method and to use it for drug monitoring. First we developed the handy sampling method of sweat secreted and simultaneously we measured the amount of sweat. The handy method of sampling was archived by using a small vial (inner volume 0.6 ml) containing few percentage alcohol-water solution. The vial attached on the surface of human skin for 3 to 5 min for collecting sweat. Then the component in sweat was analyzed by liquid chromatograph, capillary electrophoresis, GC/MS and etc.1.The variation of caffeine in sweat was measured after intake of a tablet containing caffeine. Caffeine and its metabolites were found in sweat and their concentrations were varied according to the elapsed period.2. Codeine was also found after intake of a medicine.3. Organic carboxylic acids and glucose were founded in sweat and their concentration variation were also studied after their intakes.4. Volatile organic gases are directly collected from skin surface. The gases was led to cold trap for 30 second, and switched to analysis by gas chromatography. New devices of trapping has been investigated and applied for international patents.5. Inorganic anions also investigated.From above studies, we concluded it is possible to use sweat for drug monitoring, because it contains a lot of components that have a certain relationship with the concentration in blood. The use of sweat is very attractive because it is easy to adopt for testing of body conditions by people for their control of daily life.