MicroScale Thermophoresis device

蛋白质-配体相互作用的表征是酶学和药物发现与开发的重要方法。此外，定量，即结合亲和力的确定，是特别重要的。微尺度热泳（MST）设备是表征和定量蛋白质-配体和蛋白质-蛋白质相互作用所必需的，如申请人的项目建议中所述。结合实验将为独特酶的催化机制提供新的重要见解，并将有助于基于选定的医学相关靶酶抑制剂的药物开发。对于某些项目，由于酶或配体的氧敏感性，结合研究必须在严格的厌氧条件下进行。对于所有的项目，实验必须在小批量进行，高通量是很大的优势。对于某些项目，所使用的方法必须与洗涤剂的使用相兼容。与其他方法和设备相比，mst方法和mst设备非常适合所有项目要求。以申请机构为代表，mst仪器将会在多个由政府资助的研究团体中使用，例如研究小组1976和2202、优先计划1927、卓越研究小组CIBSS和合作研究中心850。

The characterization of protein-ligand interactions represents an important method in enzymology and drug discovery and development. Moreover, the quantification, that is the determination of binding affinities, is of special importance. The MicroScale Thermophoresis (MST) device is required for the characterization and quantification of protein-ligand and protein-protein interactions as outlined in the project proposals of the applicants. The binding experiments will provide new, important insights into the catalytic mechanisms of unique enzymes and will contribute to drug development based on inhibitors of selected, medically relevant target enzymes. For some of the projects, binding studies have to be performed under strictly anaerobic conditions due to the oxygen-sensitivity of the enzymes or ligands. For all projects, experiments have to be performed in small volumes, and high throughput is of great advantage. For some of the projects, the used method must be compatible with the use of detergents. In contrast to other methods and devices, the MST-method and MST-device is ideally suited for all project requirements. Represented by the applicants, the MST-device will be used within several research consortia funded by the DFG, such as the RTGs 1976 and 2202, the Priority Programme 1927, the cluster of excellence CIBSS and the Collaborative Research Centre 850.