Immunocytochemistry on deposition and degradation of cell wall components

对杨树苯丙氨酸解氨酶（PAL）、肉桂酸4-羟化酶（C4 H）、阿魏酸5-羟化酶（F5 H）、肉桂醇脱氢酶（CAD）和芥子醇脱氢酶（SAD）的免疫细胞化学定位进行了研究。此外，还观察到了乳酸酶（LAC）的定位。使用根据其氨基酸序列合成的肽产生针对上述酶的抗体。PAL、F5 H、CAD和SAD在次生壁形成过程中定位于胞液中。而C4 H和F5 H在次级壁形成过程中定位于高尔基体和粗面内质网上。这些结果表明，苯丙烷单元的羟基化发生在高尔基体和粗面内质网上，漆酶（LAC）的免疫定位发生在次生壁形成的后期。射线薄壁组织内壁有强烈的标记。15乳酸不仅参与木质素单体的聚合反应，而且还参与了木聚糖和葡甘露聚糖的抗损伤和抗微生物侵袭的作用。木聚糖沉积在次生壁内，而葡甘露聚糖沉积在次生壁内。

Immunocytochemical localization of phenylalanine ammonia-lyase(PAL), cinnamate 4-hydroxylase(C4H), ferulate 5-hydroxylase(F5H), cinnamyl alcohol dehydrogenase(CAD), and sinapyl alcohol dehydrogenase(SAD) were investigated in poplar. In addition, localization of laocase(LAC) was observed. Antibodies against the above enzymes were raised using the peptides synthesized according to their amino acid sequences. PAL, F5H, CAD and SAD were localized in the cyto-sol during the secondary wall formation. However, C4H and F5H were localized on the Golgi apparatus and rough endoplasmic reticulum during the secondary wall formation. These results indicate that the hydroxylation of phenylpropane unit occurs in/on the Golgi apparatus and rough endoplasmic reticulum.Immunolocalization of laccase(LAC) was localized in the later stage of secondary wall formation. Intense labeling was observed at the inner wall of ray parenchyma. LAC may be involved in not only the dehydrogenative polymerization of monolignols but also the protection against the wounding and the microbial attacksImmunolacalization of xylan and glucomannan was investigated in Japanese beech and Japanese cypress. Xylan is deposited intussusceptionally to the secondary wall, though glucomannan is deposited appositionally.