MECHANISM OF INFECTION OF EPSTEIN-BARR VIRUS TO T AND NK CELLS

1)建立了外周血淋巴细胞EB病毒DNA的实时荧光定量聚合酶链式反应方法。用免疫磁珠法分离EB病毒相关性疾病患者的淋巴细胞亚群也使我们能够确定EB病毒感染的淋巴细胞亚群2)L-Meg是T细胞淋巴瘤来源的胸腺细胞系，表达CD21EBV受体，生长在Balb/c饲养层细胞上。作为EB病毒感染T细胞的模型，我们尝试用含有新霉素抗性基因的重组EB病毒感染L-Meg细胞，建立非饲养细胞非依赖细胞系。我们能够证实EB病毒感染后EBNA蛋白的表达，但G418抗性的L-Meg细胞株尚未建立。3)我们检测了急慢性EB病毒感染患者淋巴细胞亚群上趋化因子受体的表达。观察到几种趋化因子受体的上调和下调。由于所审查的案件数量仍然很少，因此需要进一步研究。

1) The method of real time PCR for EBV DNA of peripheral lymphocytes were established. Separation of lymphocyte subpopulations of the patients with EBV related diseases by the immunomagnetic bead method also enabled us to determine an EBV infected lymphocyte subset.2) L-MEG is the thymocyte derived cell line from T cell lymphoma, express CD21 EBV receptors and grows on the Balb/c feeder cells. As a model of EBV infection to T cells we tried to establish feeder cell independent cell lines of L-MEG by infection of recombinant EBV which contained neomycin resistant gene. We were able to confirm the expression of EBNA protein after infection with EBV, however, G418 resistant L-MEG cell lines were not yet established.3) We examined the expression of chemokine receptors on lymphocyte subsets of the patients with acute and chronic EBV infection. Upregulation and down regulation of the several chemokine receptors were observed. Since the number of the cases examined were still a few, further study was required.