Study of mechanism and condition for ischemic preconditioning induced by volatile anesthetics pretreatment in the liver

氟烷、异氟醚和七氟烷可减轻禁食大鼠肝脏缺血再灌注损伤，提示其可能参与了缺血预适应。在大鼠肝脏切片培养中，异氟醚减轻了低氧诱导的细胞凋亡。采用全细胞膜片钳技术研究了异氟醚对胰腺β细胞K-ATP通道的直接作用。异氟醚以剂量依赖方式抑制K-ATP通道。异氟醚重复作用可增强K-ATP通道的抑制作用。

Halothane, isoflurane and sevoflurane attenuated ischemia-reperfusion injury in the perfused liver of fasted rats, suggesting a possible involvement of ischemic preconditioning.In the rat liver slice incubation, isoflurane attenuated apoptosis induced by hypoxic challenge. Suppression of apoptosis is predominant in the hepatocyte.Direct effect of isoflurane on K-ATP channel was studied in isolated pancreatic β cells using whole cell patch clamp method. Isoflurane suppressed K-ATP channel in a dose-dependent manner. Repeated treatment of isoflurane enhanced the suppression of K-ATP channel.