Study of human exposure assessment of chemical materials in daily life and their endocrine disrupting activity.

内分泌干扰化学物质（EDCs）由影响人类和动物荷尔蒙功能平衡的合成和天然化学物质组成。一些杀虫剂和工业化学品可以通过模仿内源性激素的作用来影响动物生理。EDCs对人类健康和生殖的潜在风险尚未得到充分确定。这部分是由于缺乏准确的分析方法来量化人类生物样品中的EDCs，以及体外雌激素活性测定以评估EDCs对人类的风险。本研究建立了一种准确、灵敏、选择性的定量测定人体生物样品中双酚A （BPA）、组酚A二甘油酯（BADGE）、烷基酚、邻苯二甲酸酯和异黄酮等EDCs的分析方法。此外，我们还利用细胞增殖实验和受体竞争结合实验研究了许多合成化学物质的雌激素活性。我们已经完成了双酚a在罐装饮料和其他食品中的迁移研究，以及对蜂蜜污染可能性的调查。由于这些关于双酚a的研究及其在增加生殖功能障碍和癌症风险方面的潜在作用，对人类暴露进行评估是迫切需要的。因此，我们建立了HPLC、LC/MS、GC/MS等检测人体生物样品中BPA和BADGE代谢物的方法，并通过细胞增殖和受体结合效应来评估这些化学物质的雌激素活性。另一方面，据报道，从食品包装薄膜、医疗器械等聚氯乙烯产品中浸出了一些雌激素化学物质。因此，我们采用柱切换LC/MS联用在线萃取技术对PVC产品中EDCs进行了灵敏、选择性和准确的测定。该方法成功地用于健康人生物样品中EDCs的测定，明确了人体暴露的主要来源。少

Endocrine disrupting chemicals (EDCs) consist of synthetic and naturally occurring chemicals that affect the balance of hormonal functions in humans and animals. Some pesticides and industrial chemicals can affect animal physiology by mimicking the effects of endogenous hormones. The potential risks to human health and reproduction from the EDCs have not been well established. This is due in part to the absence of the accurate analytical methods to quantify EDCs in human biological samples and the in vitro assay of estrogenic activity for risk assessment of the EDCs to human. In this study we establish an accurate, sensitive and selective analytical method for the quantification of EDCs such as bisphenol A (BPA), hisphenol A diglycidyl ether (BADGE), alkylphenols, phthalate esters and isoflavones in human biological samples. In addition, the estrogenic activity of many synthetic chemicals was investigated by using cell proliferation assay and receptor competitor binding assay.We have a … More chieved the study of BPA migration in canned drink and other food-stuff and the investigation of the possibility of contamination of honey. Because of these studies of BPA and their potential role in increasing risk for reproductive dysfunction and cancer, the need for an assessment of human exposure is urgent. Therefore, we developed the methods for measurement of BPA and BADGE metabolites in human biological samples by HPLC, LC/MS and GC/MS, and the assays for estimation of estrogenic activity of these chemicals by cell proliferation and receptor binding effects. On the other hands, some estrogenic chemicals were reported to be leached out from such PVC products such as food wrapping films, medical devices. In this reason, we employed column-switching LC/MS coupled with on-line extraction for the sensitive, selective and accurate determination of EDCs in respet with the PVC products. This method was successfully used to determinate EDCs in human biological samples from healthy humans, and the main, source of human exposure was clarify. Less