Development of Scientific Basics of New Polymer-Drugs Based on Sugar Balls

目前的研究是以糖/脂混合糖球的合成为主题的，并阅读一个客人的分子到主机的分子。Galactose-bearing glycopeptide型糖球胶囊是设计的，但它是预期与肝脏细胞进行特别互动的。含全乙酰糖基化糖基化N羧酸盐（NCA）的聚合物反应已在室温下-30 ° C的情况下携带8-苯胺-l-萘磺酸盐（ANS）的情况下，在15分钟内被引入到终端氨基组形成羟基层。然后，该产品已被分解为甲醇中的羟基单羟基化。UV and fluorescence measurements indicated that the existence of ANS inside the sugar ball capsule.Synthesis of "sugar cylinders"， novel graft copolymers [poly]（L-Lysine）-接枝糖基肽-渗透性聚乙烯（氨基胺）牙医也是由聚合物的反应引起的。（L-Lysine）-Graft-poly（氨基胺）丁二聚体，在-30 ° C时，含过氧化物化N-乙酰-D葡萄糖胺-替代L-serine N-carboxyanhydride，聚合物反应中的终端氨基组的功能为1.0，由核磁共振光谱分析确定。糖球/等离子体DNA复合体的细胞识别能力通过利用热凝胶胶原蛋白（WGA）进行了测试。The minimum inhibitory concentrations of the sugar ball/DNA complexes were well fitted with those of corresponding sugar balls。根据限制性内核酶对复合体的分析在37 ° C时被调查。DNA dissociated by poly（aspartic acid） appeared resistant to the nucleases。

The present study is concerned with synthesis of sugar/lipid-hybrid sugar balls and reading of a guest molecule into the host molecule. Galactose-bearing glycopeptide-type sugar ball capsule was designed, which was expected to interact with liver cell specifically. Polymer reaction of poly(amido amine) dendrimer with peracetylated sugar-substituted serine N carboxyanhydride (NCA) was carried out in the presence of 8-anilino-l-naphthalenesulfonate (ANS) in chloroform at -30 ゜C for 15 min. Acyl groups were introduced to the terminal amino groups to form a hydrophobic layer.Then, the product was deacetylated with hydrazine monohydrate in methanol. UV and fluorescence measurements indicated that the existence of ANS inside the sugar ball capsule.Synthesis of "sugar cylinders", novel graft copolymers [poly(L-lysine)-graft glycopeptide-persubstituted poly(amido amine) dendrimer] was also carried out by the polymer reaction of poly(L-lysine)-graft-poly(amido amine) dendrimer with peracetylated N-acetyl-D glucosamine-substituted L-serine N-carboxyanhydride in chloroform at -30 ゜C, Functionality of the terminal amino groups in the polymer reaction was 1.0, which was determined by NMR spectro scopy.Cell recognition ability of the sugar ball/plasmid DNA complexes was examined by erythrocyte agglutination inhibition assays using wheat germ agglutinin (WGA). The minimum inhibitory concentrations of the sugar ball/DNA complexes were well fitted with those of corresponding sugar balls. The nuclease digestion of the complex by restriction endonucleases was investigated at 37 ゜C. DNA dissociated by poly(aspartic acid) appeared resistant to the nucleases.