Glucose metabolism and Molecular basis analysis for immunoprotective mechanisms angainst Babesia spp. Infection

1)葡萄糖代谢：利用建立的短期培养体系，研究了微巴贝虫和罗德海贝虫感染红细胞的葡萄糖摄取系统。根据标记的d -葡萄糖摄取，两种感染的红细胞都显示出新的葡萄糖运输系统，这显示了两种巴贝虫原虫的不同特征。形态学检查，两种感染的红细胞均有特异性改变。2)抗原生动物药物的开发：基于糖代谢相关酶活性的结果，对一些抗原生动物药物的候选设计进行了成像。然而，没有药物被开发出来。3)原生动物膜蛋白分子分析：根据感染红细胞表面膜蛋白分子分析结果，检测到脾巨噬细胞中有一种蛋白促进IL-12的产生。但其完整序列尚未建立。4)原生动物的免疫保护机制：Th1细胞的分化是预防原生动物感染的重要因素。两相分泌il - 12p70与这种分化密切相关。然而，除il - 12p70外，还需要其他机制来消除原生动物。5)生物反应修饰剂的研制：检测感染红细胞培养上清中的热稳定蛋白。由于该蛋白能显著提高脾巨噬细胞IL-12的产生，因此被认为是生物反应修饰剂的候选之一。

1) Glucose metabolism : Using short term cultivation system established, glucose uptake system was investigated in Babesia microti and B. rodhaini infected erythrocytes. Based on the labeled D-glucose uptake, both infected erythrocytes showed new glucose transport system, which revealed different character between two Babesia protozoa. On the morphological examination, specific changes observed in both infected erythrocytes.2) Development of anti-protozoan drugs : Based on the results of glucose metabolism related enzyme activities, some candidate designs of anti-protozoan drugs was imaged. However, no drug was developed.3) Molecular analysis for protozoan membrane proteins : Based on the results of molecular analysis for surface membrane proteins of infected erythrocytes, one protein enhanced IL-12 production from splenic macrophage was detected. However, full sequence of its was not established.4) Immunological protective mechanism for protozoa : Differentiation for Th1 cells was an important factor for prevention of protozoan infection. Two phase secretion of IL-12 p70 was closely related to this differentiation. However, other mechanism beside IL-12 p70 was necessary for the elimination of protozoa.5) Development of the biological response modifier : The heat stable protein in culture supernatant of infected erythrocytes was detected. Since this protein remarkably enhanced IL-12 production from splenic macrophage, iit was considered that one of the candidate for biological response modifier.