Development of bioremediation of remaining dioxins using Sea elegans

二恶英是一种内分泌干扰物，近年来已被认为是环境污染物中诱变的功能失调因素，并影响胎儿的发育和分化。本研究旨在建立一套利用基因工程技术对环境中二恶英进行检测和生物修复的系统。因此，我们成功地开发了环境中二恶英的便捷测量系统。该系统对二恶英的检测范围为10fmol ~ 100pmol。为了构建二恶英生物修复体系，从枯草芽孢杆菌（Bacillus subtilis）和黄孢平革菌（Phanerochaete chrysosporium）中克隆了降解二恶英的基因和酶的cdna。这些酶在大肠杆菌中进行了表达，并利用上述建立的报告基因法测定了表达酶对二恶英的降解活性。结果表明，部分酶具有降解二恶英的活性。本研究结果有望为环境中残留二恶英的生物修复技术的建立做出贡献。

Recently, environmental pollutant by dioxins, one of the endocrine disrupters has been considered as dysfunctional factor of mutagenesis, and to affect on development and differentiation of fetals. In this study, to confirm the system of measurement and bioremediation of dioxins in environmental by using of genetic engineering.As results, we have successful developed convenience measurement system of dioxins in environment. The detection range of dioxins using the developed reporter assay system showed 10 fmol to 100 pmol. To consruct the system of bioremediation of dioxins, the genes and cDNAs of enzymes that were expected to degrade the dioxins, has been cloned from Bacillus subtilis and Phanerochaete chrysosporium. The enzymes were expressed in Esherichia coli, and the dioxin degraded activity of the expressed enzymes was estimated by using the developed reporter assay above mentioned. From the results, some enzymes showed the activity of degradation of dioxins. These results obtained in this study are expected to contribute the establishment of bioremediation of remaining dioxins in environment.