Production of hypoxic adaptation model in rat

所有的实验(杂交、怀孕、哺乳和锻炼)都是在无菌、低氧的室内进行的。4周龄Wistar雄性Wistar大鼠4周龄随机分为3组：低氧运动组(MHE)、低氧安静组(MHS)。男性和女性运动组被允许自由使用转轮。使用每天读取的机械计数器记录车轮的旋转次数(1.16米，周长)。选择8周内跑总距离最大的雄性和雌性大鼠。选择雄性和雌性大鼠进行杂交。出生后第21天，这些孩子(第一只高海拔天然大鼠；F1)与他们的母亲分开。将F1代雄性大鼠随机分为3组：MHE-F1组、MHS-F1组和常氧安静低海拔原生大鼠作为对照组。在18周龄时，P1、F1、F3(第三高海拔NA…用乙醚麻醉F5(第5只)和F7(第7只)大鼠，分别从主动脉、脑、心、肝、横隔肌、骨骼肌(比目鱼肌和腓肠肌)处死。将整个大脑分为以下四个区域：小脑、额叶、纹状体、海马区。研究(1)：与其他组相比，高原原住民低氧运动组的额叶、小脑和纹状体的单胺类物质水平显著降低。这些研究表明，低氧运动降低了整个大脑中的单胺水平，并且对低氧和/或运动的敏感性因大脑区域的不同而不同。研究(2)三组之间隔膜和比目鱼肌的去甲肾上腺素水平没有显著差异。这项研究阐明，低氧引起情人交感神经反应减弱，但它会导致心脏和腓肠肌白色肌肉反应增加。以上结果提示，大鼠组织对长期低氧的交感反应发生了改变。研究(3)本研究证实，中度低氧和低氧训练可减轻肝脏DNA损伤，降低肝脏GSH水平和GR活性。这些结果表明，中度低氧和低氧训练可减轻氧化应激。较少

All experiments (hybrid, pregnancy, nurse and exercise) were carried out in the sterile, hypoxia chamber. At the age of 4 weeks, the Progenitor's group (PI) Wistar male and female rats were randomly assigned to the following groups : male rats : hypoxia and exercise (MHE), hypoxia and sedentary (MHS). The male and female exercising groups were allowed free access to the running wheel. The numbers of revolution of the wheel (1.16m, circumference) were recorded by using a mechanical counter that was read daily. Male and female rats who did the highest total amount of running distance during 8 weeks were selected. The selected male and female rats were hybridized. The children (the first of high-altitude native rats ; F1) were separated from their mother after postnatal day 21. The male rats of F1 were randomly divided into three groups : MHE-F1, MHS-F1 and normoxic and sedentary low-altitude native rats, as control group. At the age of 18 weeks, the P1, F1, F3 (the third high-altitude na … More tive rats), F5(the fifth) and F7(the seventh) were anesthetized by ether and sacrificed by sampling of blood from the aorta, and brain, heart, liver, diaphragm, skeletal muscle (soleus and gastrocnemius white muscle). The whole brain was divided into the following four regions : cerebellum, frontal lobe, striatum, hippocampus.Study (1) : Monoamines levels of the hypoxic exercise group in high-altitude native significantly decreased in the frontal lobe, cerebellum and striatum, compare with the other groups. These study suggest that hypoxic exercise decreased monoamine levels in whole brain, and that sensitivity to hypoxia and/or exercise differed according to brain regions.Study (2) No significant differences were found in the norepinephrine levels for the diaphragm and soleus muscle among the three groups. This study clarified that hypoxia elicits a decreased sympathetic response in the lover, However, it cause an increase in the response in the heart and gastrocnemius white muscle. These results suggest that the sympathetic response to long -term hypoxia changes with the rat tissues.Study (3) The present study confirmed that moderate hypoxia and hypoxic training induce attenuate the liver DNA damage and decrease the liver GSH level and GR activity. These results indicate that moderate hypoxia and hypoxic training result in deceased oxidative stress. Less