Gene analysis familially condensed juvenile diabetics developed sporadically in Indonesia

采用PCR-PFLP方法分析了印度尼西亚及其邻近的巴布亚新几内亚居民的糖尿病和肥胖易感基因的多态性，结果表明：（1）在印度尼西亚（1）UCP-1基因-112 A/C多态性与肥胖无关，但爪哇岛居民的C等位基因频率高于巴厘岛居民，与日本人相当。（2）PPARγ2基因Pro 12 Ala多态性频率低于白种人的12，巴厘岛本地人为零。（3）未发现线粒体DNA 3243 A →G突变。（4）脂联素基因SNP 45 T →G多态性与巴厘岛土著居民肥胖及糖耐量相关。然而，SNP 276多态性没有揭示这种关联。（5）UCP-3基因-55C C T多态性与巴厘岛和伊里安查亚土著人群的肥胖相关，也与糖耐量相关。(2)在巴布亚新几内亚（1）糖原合成酶轴突10的MetA 16 Val多态性与肥胖无关，但其频率高于日本人。（2）未检测到PPARγ2基因Pro 12 Ala多态性。（3）肥胖者β2肾上腺素能受体基因Trp 64 Arg多态性频率较高。（4）UCP-1基因-112 A → C突变很少检出，Met 229 Leu多态性未检出。（5）UCP-3基因-55 C/T多态性与肥胖无关。（6）TNFα基因未发现-308G A和-238G A突变。（7）脂联素基因SNP 276 T →G突变和G等位基因频率与肥胖无关，但高于日本人，表明印尼和巴布亚新几内亚不同民族和不同地区人群的糖尿病和肥胖易感基因多态性频率不同。此外，这些国家近年来糖尿病发病率的增加似乎不仅是由于生活方式的改变，而且由于易感基因的存在。

Polymorphisms of diabetos-and obosity-susceptible genes were analysed using PCR-PFLP method in inhabitants of Indonesia and its vecinal Papua New Guinea.The results were as follows :(1) In Indonesia(1)-112A→C polymorphism of UCP-1 gene was not associated with obesity, but its C-allele frequency of Java islanders was higher than that of native inhabitants of Bali, and comparable to that of Japanese.(2)Frequency of Pro12Ala polymorphism of PPARγ2 gene was lower than one half of that of Caucasians, and that of native inhabitants of Bali was null.(3)3243A→G mutation of mitochondrial DNA was not found.(4)SNP45T→G polymorphism of adiponectin gene was associated with obesity in native people of Bali, and also with glucose tolerance. However, SNP276 polymorphism did not reveal such association.(5)-55C→T polymorphism of UCP-3 gene was related to obesity in native people of Bali and Irian Jaya, and also with glucose tolerance.(2) In Papua New Guinea(1)MetA16Val polymorphism of axon 1O of glycogen synthase had no association with obesity but its frequency was higher than that of Japanese.(2)Pro12Ala polymorphism of PPARγ2 gene was not detected.(3)Frequency of Trp64Arg polymorphism of β2 adrenergic receptor gene was higher in obese subjects.(4)-112A→C mutation of UCP-1 gene was detected rarely, while Met229Leu polymorphism was not.(5)-55C→T polymorphism of UCP-3 gene was not associated with obesity.(6)-308G→A and -238G→Amutation of TNFα gene was not observed.(7)Frequency of SNP276T→G mutation and G-allele of adiponectin gene was not related to obesity but was higher than that of Japanese.These results indicate that frequency of diabetes-and obesity-susceptible gene polymorphisms varies among ethnic groups and people inhabiting in different regions in Indonesia and Papua New Guinea. Besides, the recently increasing incidence of diabetes in these countries appears to be due to not only the change of life-style through rapid Westernization but possession of the susceptible genes.