Biosyntheis of volatile C-13 norisoprenoid

1.大马士革玫瑰磨坊花中的大马士革酮祖先。从大马花中分离到两个大马花酮祖细胞。它们的化学结构被确定为巨茄-6,7-二-3,5,9-三醇9-Ο-β- d -葡萄糖苷。每个化合物的立体化学也通过直接比较它们的核磁共振光谱和在hiplc上的色谱谱图来确定。绿茶提取物中的大马松酮在生产罐装或瓶装绿茶饮料的绿茶提取物的灭菌过程中，会产生大马松酮作为异味化合物。经分离鉴定为乙酰二醇和丙烯三醇糖苷。它们的立体化学也通过与合成样品的比较来确定。我们还提出了这些化合物转化为大马士革酮的途径。类胡萝卜素裂解酶参与c13 -类去异戊二烯挥发性化合物的形成c -13 -类去异戊二烯挥发性化合物推测是由类胡萝卜素如β-胡萝卜素、新黄嘌呤等在植物组织中通过类胡萝卜素裂解酶的作用产生的，例如一些水果和花朵。我们首次从杨桃（Averrhoa carambola）和绿茶叶子中鉴定出一种酶。Winterhlater教授小组从动物和木瓜果实中鉴定了类似的酶。我们不仅对b-胡萝卜素的生化特征进行了表征，而且对酶促反应产物进行了表征。

1.Damascenone progenitors in the flowers of Rosa damascena Mill.Two damascenone progenitors have been isolated from the flowers of R. damascena Mill. Their chemical structures were determined to be megastigma-6,7-dien-3,5,9-triol 9-Ο-β-D-glucolyranosides. The stereo chemistry for each compound was also determined by the direct comparison of their NMR spectra as well as chromatographic profiles on HIPLC.2.Damascenone progenitors in green te extractsDuring the course of sterilization of green tea extract for production of canned or bottled green tea drinks, damascenone was generated as off-flavor compound. The progenitors were isolated and identified to be acetylenic diol and allenic triol glucosides. Their stereo chemistries were also determined by comparison with synthetic specimens. We have also proposed the conversion routes for these compounds to damascenone.3.Carotenoid cleavage enzymes being involved in the formation of C13-norisoprenoidsC-13 Norisoprenoid volatile compounds are speculated to be generated from carotenoids such as β-caroten, neoxantin by the action of carotenoid cleavage enzymes in plant tissues, such as some fruits and flowers. We have, for the first time, characterized an enzyme from star fruit (Averrhoa carambola) and green tea leaves. Similar enzymes were characterized from animals and quince fruits by the group of Prof. Winterhlater. We have characterized not only the biochemical profiles, but also the products on the enzymatic reaction from b-carotene.