GnRH in fish leukocytes

基因组非常小的河豚被发现有三种类型的GnRH，即，sbGnRH、sGnRH和cGnRH II以及三种GnRH受体（GnRH-R）。我们通过cDNA克隆确定了它们的一级结构。RT-PCR分析表明sbGnRH和sGnRH在包括淋巴器官和白细胞在内的多种组织中表达，而cGnRH II除了在脑中表达外，仅在肾、头肾和脾中表达，这表明GnRH-R在免疫器官和白细胞等多种组织中均有表达。因此，GnRH在免疫系统中的重要性已得到明确认识。检测了三种类型的GnRH对COS细胞上表达的三种GnRH-R的亲和力，发现所有三种受体对sGnRH和cGnRH II的亲和力均高于对sbGnRH的亲和力。这些结果表明GnRH通过GnRH-R发挥多种功能，对淋巴细胞进行分类对于分析GnRH在免疫系统中的功能至关重要。本文测定了淋巴细胞的几种免疫因子，如CD_3、CD_4、CD_8、IL-8、IL-12、TCRγ、IgM、IgD等，并分析了PHA、ConA、Poly I：C、LPS、PMA等免疫调节剂对淋巴细胞免疫因子，如GnRH等的影响，同时分析了生殖内分泌系统与白细胞功能的关系。本文还讨论了GnRH对酪氨酸磷酸酶表达的下调作用

Fugu, which has very small genome size, was found to have three types of GnRH, i.e., sbGnRH, sGnRH and cGnRH II, and three GnRH receptors (GnRH-R). We determined their primary structures by cDNA cloning. RT PCR analyses showed that sbGnRH and sGnRH are expressed in various tissues including lymphoid organs and leukocytes, while cGnRH II is expressed only in the kidney, the head kidney and the spleen in addition to the brain, indicatin. Expression of GnRH-R was also recognized in variety of tissues including immune organs and leukocytes. Significance of GnRH in immune system has thus been clearly recognized. Each Affinity of the three types of GnRH against the three GnRH-R expressed on COS cells was tested, and found that all three receptors showed higher affinities against sGnRH and cGnRH II, than against sbGnRH. These results indicate a large variety of the GnRH functions via GnRH-R.It is essential to classify the lymphocytes for the analysis on the function of GnRH in the immune system. We determined several immune factors of lymphocytes such as, CD3, CD4, CD8, IL-8, IL-12, TCRγ, IgM, and IgD, and now analyzing the effects of immunomodulators, such as PHA, ConA, Poly I : C, LPS and PMA, against those immune factors of lymphocytes, including GnRH.In addition to these observations, analyses on the relationships between reproductive endocrine system and leukocyte functions. Downregulation of the expression of tyrosine phosphatases by GnRH was also discussed