Stage- and Organ-specific Regulation of Plant Hormone Abscisic Acid Biosynthesis in Plants

脱落酸（Abscisic acid， ABA）是一种植物激素，在植物生长发育的许多方面发挥重要作用，包括种子的成熟和休眠以及对各种环境胁迫的适应。因此，了解内源ABA水平的调控机制对于阐明ABA在植物生长和生理反应中的作用至关重要。近年来，利用ABA缺陷突变体克隆基因的研究揭示了ABA主要生物合成途径的细节，以及基因家族的存在和各自的器官特异性表达。因此，现在我们站在这一点上，能够看到ABA生物合成调节的精确机制在每一个ABA相关的现象。本研究探讨了拟南芥醛氧化酶3 （AAO3）基因在体内和体内与ABA生物合成相关的功能，以及ABA生物合成的复杂调控机制。我们还尝试利用AAO3启动子：AAO3- gtp融合来研究AAO3蛋白的定位。

Abscisic acid (ABA) is a plant hormone that plays an important role in many aspects of plant growth and development, including seed maturation and dormancy as well as adaptation to a variety of environmental stress. Thus an understanding of the regulation mechanisms of endogenous ABA levels is crucial for elucidation of ABA action in plant growth and physiological responses. Recent studies based on the cloning of genes using ABA-deficient mutants have reveals details of the main ABA biosynthetic pathway, and the presence of gene families and their respective organ-specific expressions. Thus, now we are standing at the point to be able to view the precise mechanism of the regulation of ABA biosynthesis in each ABA-related phenomenon. In this study, in vivo and in vivo function of Arabidopsis aldehyde oxidase 3 (AAO3) gene in relation to ABA biosynthesis, and with the results complex regulation of ABA biosynthesis has been discussed. We have also tried to investigate the localization of AAO3 protein, using AAO3 promoter: AAO3-GTP fusion.